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What is the effect if the paper is allowed to remain in the chamber after the solvent front has reached the top of the plate in a chromatographic work?
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What problem will ensue if the level of the developing liquid is higher than the applied spot in a TLC analysis?
The effect on chromatographic work if the solvent level in the developing chamber is higher than the spotted sample is a thin layer chromatography. The solvent becomes polar enough and spot will move some distance.
What can you use to separate mixtures of identify compounds?
Some methods are: distillation, ion exchange, filtration, sedimentation, solvent extraction, precipitation, chromatographic procedures and many others.
Why must the chromatography chamber be closed tightly?
so that the atmosphere in the jar becomes saturated with solvent.
What happens to particles during chromatography?
Particles present in the dye to be separated is dissolved by the solvent and then carried throught the chromatographic paper, the extent to which each die will travel will depent on the amount of that die is present in the sample die.
What is The ability of a solvent to dissolve a given solute?
Dissolving power refers to the ability of a solvent to dissolve a certain amount of a solute.Once no more solute can be dissolved in a solvent it is said to be reached to its saturation point. By changing temperature and pressure of a solution that has reached its saturation point, some more solute can be dissolved and thereby make a supersaturated solution.
A method of separating mixtures by using chromatographic paper and a solvent?
This method is called paper chromatography.
What problem will ensue if the level of the developing liquid is higher than the applied spot in a TLC analysis?
The effect on chromatographic work if the solvent level in the developing chamber is higher than the spotted sample is a thin layer chromatography. The solvent becomes polar enough and spot will move some distance.
What are the considerations in choosing a chromatographic solvent?
One of the considerations of a chromatographic solvent is whether it will bring out the different Rf values of the samples that are being used. If all of the compounds have the same Rf values then the separation of pigments would have failed due to the fact that all of them are in the same spot.
What forces the eluting solution to move along the chromatographic support material?
An eluting solvent is then allowed to move up the plate by capillary action, drawing the components of the mixture along by varying degrees.Read more: chromatography
What can you use to separate mixtures of identify compounds?
Some methods are: distillation, ion exchange, filtration, sedimentation, solvent extraction, precipitation, chromatographic procedures and many others.
Why must the chromatography chamber be closed tightly?
so that the atmosphere in the jar becomes saturated with solvent.
What happens to particles during chromatography?
Particles present in the dye to be separated is dissolved by the solvent and then carried throught the chromatographic paper, the extent to which each die will travel will depent on the amount of that die is present in the sample die.
What is The ability of a solvent to dissolve a given solute?
Dissolving power refers to the ability of a solvent to dissolve a certain amount of a solute.Once no more solute can be dissolved in a solvent it is said to be reached to its saturation point. By changing temperature and pressure of a solution that has reached its saturation point, some more solute can be dissolved and thereby make a supersaturated solution.
Why is it important that the sample spots are not below the solvent level when the filter paper is first placed in the developing chamber?
By placing the spot above the level of the solvent in the beaker, you slow the flow of solvent which results in improved separation of the components in the spot. If you placed it below the level of the solvent in the beaker, the solvent would just flow at a much faster rate and instead of separating the components in the spot, they would simply be washed away.
Why stop paper chromatography from reaching the top of the paper?
The end of the paper nearest the spot is allowed to be in contact with a suitable solvent, WITHOUT immersing the spot itself. If the spots were immersed they would dissolve in the solvent and be pulled down into the solvent itself.
How can you dissolve more solid into liquid?
Once you have reached a point where you cannot dissolve any more solute in solvent, you have reached a saturation point of the liquid. Now if you increase the temperature of the solution you will be able to dissolve some more solute. Another way to do this is to greatly increase the volume of solvent.
What would be the consequence of having so much solvent in the developing chamber that it would cover the application?
If the solvent level in the developing jar is deeper than the origin (spotting line) of the TLC plate, the solvent will dissolve the compounds into the solvent reservoir instead of allowing them to move up the plate by capillary action. Thus, you will not see spots after the plate is developed. These photos show how the yellow compound is running into the solvent when lifted from the developing jar.
