The effect of substrate concentration on the rate of reaction of h202 and catalase?

Answer 1

As the substrate concentration increases, so will the enzyme activity and hence there will be a quick reaction. however, only up to a certain point ( where, if you drew a graph of the reaction, the line will level off ) as all the active sites in the enzyme are occupied and the reaction cannot go any faster. Here more enzymes will be needed to speed up the reaction.

Answer 2

As the concentration of substrate decreases, due to its conversion to product(s), the rate of the reaction will slow. This is due to the less and less frequent random encounters between substrate and enzyme. As more of the peroxide is converted to oxygen and water, there is less remaining to interact with the enzyme; therefore, the reactions slows.

Answer 3

Most enzyme reaction rates are calculated using a steady state assumption, given that the enzyme concentration typically doesn't change once the reaction is started, The only effect increasing enzyme concentration has is to increase the initial rate of the reaction, until the avaiable enzyme concentration shifts to the concentration of the enzyme-substrate complex. Reference any text on Michaelis Menten Kinetics to see the derivation.

Answer 4

different enzymes require different PH to function effectively, PH too high or low may destroy the enzyme. increasing the substrate amount will increase the resulting products within a time period, this is limited though by the amount of enzyme.

Answer 5

As substrate concentration increases, the rate of reaction between hydrogen peroxide (H2O2) and catalase also increases initially due to more substrate molecules colliding with enzyme active sites. However, at a certain point, the rate of reaction plateaus as all enzyme active sites become saturated and adding more substrate does not increase the reaction rate further. This is known as the enzyme substrate saturation point.