removal of polyphenols
salt binds to molecules to keep it from clumping
Sodium perchlorate is used to deproteinize your DNA preps. At high concentrations, Na perchlorate will remove SDS and associated proteins and prevent proteins from precipitating with your nucleic acid in your ethanol ppt step.
phenol helps to remove non polar proteins and lipids from the solution
phenol helps to remove non polar proteins and lipids from the solution
Deoxyribonucleic acid (DNA) is two strands of Ribonucleic acid (RNA) that have been put together. See http://nobelprize.org/educational_games/medicine/dna/index.html for a more detailed answer.
Trichloroacetic acid is used for precipitation of the DNA during its extraction.
To achieve precipitation DNA.
chelating Mg2+
DTT is the reducing agent for thiolated DNA
It solubalize lipids and protiens to remove them from DNA
In a DNA extraction, the purpose of a buffer is to solubilize DNA as well as RNA. Because of this, it prevents the DNA for degrading.
Boric Acid is an extraction buffer used in the isolation of DNA and when it is employed with a correct pH then it can help in getting rid of the cell components without disturbing the cell organelles i.e it retains the organelles.
ammonium acetae use to percipitate DNA from water.
to remove excess phenol from DNA to remove excess phenol from DNA
Triton X-100 is used as a lysis buffer for DNA separation.
salt binds to molecules to keep it from clumping
The buffer AP1 is vital in DNA extraction as it acts as a cleanser to break up the lipids surrounding the cellular membrane. The buffer also maintains the right environment for the DNA so it is not damaged during the extraction process.