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Q: What is the function of calcium chloride in the fractionation buffer?
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What is sorensens buffer?

Phosphate buffered saline is a buffer solution commonly used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulations, potassium chloride and potassium phosphate. The osmolarity and ion concentrations of the solutions match those of the human body.


What is the need for an ammonical buffer solution in the determination of hardness of water by edta titration?

during the complexometric titration using edta it is very necessary to maintain the ph of the solution near about 10 so we use ammonium chloride buffer if we will not use this buffer dring the titration ph of sol. will ho lower side


What is function of Buffer AP1 in DNA extraction?

The buffer AP1 is vital in DNA extraction as it acts as a cleanser to break up the lipids surrounding the cellular membrane. The buffer also maintains the right environment for the DNA so it is not damaged during the extraction process.


What are the characteristics of a buffer system?

Buffering system is the physiological system that function to keep pH within normal limits.


How are buffers produced?

There are two types of Buffer solution and both have different preparation:Acidic BufferAcidic buffers are made by mixing a weak acid with its conjugate base.Example:When we mix Acetic acid with Sodium citrate, an acidic buffer is formed.Basic BufferBasic buffers are made by mixing a weak base with its conjugate base.Example:When Ammonium hydroxide is mixed with Ammonium chloride, a basic buffer is formed.

Related questions

Why using phosphate buffer and magnesium sulphate and calcium chloride and ferric chloride for dilution water in BOD5 test?

magnesium sulphate, calcium chloride and ferric chloride are added to provided optimum environment and phosphate buffer is added to slow down the rate of death of microbes.


What is the function of sucrose in the homogenization buffer during fractionation of cells?

Sucrose is used to partially dehydrate organelles (thus keeping them intact) in homogenization buffers.


Is calcium carbonate a buffer?

Yes. Calcium carbonate is a salt and is formed from calcium reacting with carbonic acid. The salts of weak acids function as buffers. Carbonic acid is a weak acid.


Is the carbonate or the calcium the buffer in calcium carbonate?

The carbonate. Calcium is neutral.


What are the function of buffer?

the function of a buffer is to maintain the pH of the sample.


Function of a frame buffer in computers?

function of a frame buffer in computer?


Why you use ammonium chloride and ammonium carbonate in lysis buffer for DNA extraction from blood?

In lysis buffer, the function of ammonium chloride is to determine the red blood cells in samples. These samples also contain white blood cells.


What is the function of MOPS buffer RNA isolation?

It act as a buffer in Northern blotting.


What is the function of TNE buffer?

We use it for isolation of proteins from yeast cells as a lysis buffer


Why do you add iron III chloride buffer to the salicylate solution?

because you need to


What are some examples of a buffer?

Some examples of a buffer are mixture of ammonium hydroxide with ammonium chloride & mixture of acetic acid and sodium acetate.


What is the function of PBS buffer?

The main function of PBS (phosphate buffer saline) is to maintain the pH of the solution. by Himanshu Goel Jaypee University of Information Technology