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it enhance the reaction
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Role of magnesium chloride in PCR?
mgcl2 acts as a cofactor for the DNA polymerase used and is necessary for efficient functioning of the pcr
What is the function of tris hcl in PCR buffer?
IT act as a buffering agent to maintain the PH of a PCR
105 g of MgCl2 contains how much mol MgCl2?
MgCl2=95grams per mole so 105g/95g is 1.10 mols of MgCl2
What is the molarity of 80g magnesium chloride dissolved in 250ml of water?
MgCl2 Molarity = moles of solute/Liters of solution ( 250 ml = 0.250 L ) Get moles MgCl2 80 grams MgCl2 (1 mole MgCl2/95.21 grams) = 0.8402 moles MgCl2 Molarity = 0.8402 moles MgCl2/0.250 Liters = 3.4 M MgCl2 ----------------
What is the correct way to write the formula for magnesium chloride?
The synthesis equation for MgCl2 is Mg + Cl2 -> MgCl2. The decomposition reaction is MgCl2 - heat-> Mg + Cl2.
Role of magnesium chloride in PCR?
mgcl2 acts as a cofactor for the DNA polymerase used and is necessary for efficient functioning of the pcr
What is the function of EDTA in PCR?
It Inhibits the PCR reaction by chelating the magnesium ions.
What is the function of tris hcl in PCR buffer?
IT act as a buffering agent to maintain the PH of a PCR
How does a DNA molecule act as a template?
Template DNA is a DNA you want to amplify. So you should know what you are amplifying before a PCR or you can make it by sequencing your PCR product.
Role of additives in PCR?
Reactants: (dNTPs, template DNA (to be amplified), primers(bind to DNA to begin elongation of strand), DNA Polymerase (elongate DNA), & MgCl2) in buffer + H2O
What are the different types of polymerase chain reaction techniques?
types of pcr: AFLP -PCR. Allele-specific PCR. Alu-PCR. Assembly -PCR. Assemetric -PCR. Colony -PCR. Helicase dependent amplification. Hot start pCR. Inverse -PCR. Insitu -pCR. ISSR-PCR. RT-PCR(REVERSE TARNSCRIPTASE). REAL TIME -PCR
Why is MgCl2 a variable in PCR?
Mg2+ complexes with the single stranded DNA that is to be amplified, and becomes the substrate of DNA polymerase. In other words, it helps in the binding of primer (and the subsequent target DNA) to the template DNA. Different volume of Mg2+ exert different complex-forming capabilities, and thus affects the end product of PCR.
What is the role of mgcl2 in the lysis buffer?
MgCl2 is added to the lysis buffer since Mg2+ ions are co-factors for the enzyme used in the lysis buffer. This enzyme requires magnesium ions in order to function properly.
What is the purpose of the buffer in PCR?
The purpose of the buffer in PCR, I assume you talking about the 5 or 10 times PCR buffer that is provided with enzyme. Buffer is needed to give the correct pH and pottasium ion concentration for the DNA polymerase enzyme (usually DNA polymerase from Thermus aquaticus) to function.
What is pcr and types of pcr?
PCR is a biotechnological method to amplify your gene (DNA) of your interest. It produce millions of your DNA fragments hence used in cloning. There are variants of this method using the same thermocycling principle such as touch down PCR, gradient PCR, RFLP, multiplex PCR, Q PCR, RT PCR and so on.
What is the common name for mgcl2?
MgCl2 is magnesium chloride.
105 g of MgCl2 contains how much mol MgCl2?
MgCl2=95grams per mole so 105g/95g is 1.10 mols of MgCl2
