0
What else can I help you with?
What is the function of the mobile phase for developing a chromatogram?
The mobile phase in chromatography is responsible for carrying the sample through the stationary phase. It helps separate the components of the sample by their different affinities for the stationary phase. The composition and flow rate of the mobile phase can be adjusted to optimize separation.
What are mobile phase and stationary phase?
stationary phase is the solid called the filter paper and mobile phase is the liquid or organic solvent present in the developing tank or beaker by ayesha zulfiqar
What are the principles and methods of high performance liquid chromatography and gel chromatography?
Liquid chromatography separation of multicomponant system according to diffrent interaction of dissolved componant with stationary phase. the stationary phase and mobile phase is very wide range
What is difference bet GLC and HPLC?
GLC has a stationary liquid phase and gas moving phase HPLC had a stationary solid phase and liquid moving phase HPLC is done under high pressure. HPLC can be used for thermally unstable compounds as opposed to GLC HPLC can be used for polar or low volatile compounds as opposed to GLC
What called mobil phase?
The mobile phase is the liquid or gas that flows through the chromatography system, carrying the sample to be separated. It moves over the stationary phase (solid or liquid) and helps separate the components based on their interactions with the stationary phase. The choice of mobile phase depends on the type of chromatography being performed.
What is the difference between partition chromatography and adsorption chromatography?
The partition chromatography involves separation between liquids while adsoption chromatography involves solid and liquid separations. Answer: Partition Chromatography is a liquid liquid extraction which involves two solvents while adsorption chromatography is a liquid solid extraction which involves a solid stationary phase & a liquid mobile phase.
What is stationary phase and mobile phase?
mobile phase is the phase that consist of the analyte and stationary phase is the phase that is standstill
What is the requirement of stationary phase?
The stationary phase in chromatography must be a solid or liquid material that does not move during the separation process. It interacts with the sample components to separate them based on their different properties, such as polarity or size. The choice of stationary phase is critical in determining the separation efficiency and selectivity of the chromatographic method.
What process that uses pressure to separate substances in a solution?
The process you are referring to is likely a type of chromatography, known as high pressure liquid chromatography (HPLC). In HPLC, a liquid mobile phase is passed through a column of stationary phase under high pressure, separating the components of a mixture based on their interaction with the stationary phase.
How does layer chromatography work?
In layer chromatography, a mixture of substances is separated as it moves along a stationary phase, which can be a solid or liquid, due to differences in the affinities of the components for the stationary and mobile phases. The components travel through the stationary phase at different rates, leading to their separation based on their interactions with the stationary phase. By analyzing the distances traveled by the components, their presence in the mixture can be detected.
What does TLC use as its moving phase?
TLC (thin layer chromatography) uses a stationary phase such as silica gel or alumina on a glass or plastic plate. The mobile phase, which moves through the stationary phase, often consists of a solvent mixture that carries the compounds being separated.
What is liquid used in chromatography called?
The liquid used in chromatography is called the mobile phase. It is responsible for carrying the sample through the stationary phase, allowing for the separation of components based on different properties like solubility and polarity.
