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What is the function of ta polymerase buffer containing magnesium?
Wiki User
∙ 12y ago
it helps keep the ph stable
Wiki User
∙ 12y ago
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What is the function of tris and EDTA buffer?
solubilize DNA
Why using phosphate buffer and magnesium sulphate and calcium chloride and ferric chloride for dilution water in BOD5 test?
magnesium sulphate, calcium chloride and ferric chloride are added to provided optimum environment and phosphate buffer is added to slow down the rate of death of microbes.
What is the word equation for magnesium hydroxide neutralising hydrochloric acid in your stomach?
Milk of magnesia is magnesium hydroxide or Mg(OH)2 and is a base stomach acid is a dilute solution of hydrochloric acid or HCl, a strong acid. They will react chemically to produce magnesium chloride an water. MgOH + 2HCl --> MgCl2 + 2H2O
What is the function of tris hcl in PCR buffer?
IT act as a buffering agent to maintain the PH of a PCR
Can hydrofluoric acid and sodium fluoride function as a buffer?
Yes. HF is an Acid and NaF is a base they both share the common ion effect.
What is the purpose of the buffer in PCR?
The purpose of the buffer in PCR, I assume you talking about the 5 or 10 times PCR buffer that is provided with enzyme. Buffer is needed to give the correct pH and pottasium ion concentration for the DNA polymerase enzyme (usually DNA polymerase from Thermus aquaticus) to function.
What is the role of mgcl2 in the lysis buffer?
MgCl2 is added to the lysis buffer since Mg2+ ions are co-factors for the enzyme used in the lysis buffer. This enzyme requires magnesium ions in order to function properly.
Why are there nucleotides A T G and C in the master mix and what are the other components and what are there functions?
The nucleotides also called dNTPs are free nucleotides that are used in the replication of new strands. The complete master mix contains Taq DNA polymerase, deoxynucleotides, oligonucleotide primers, magnesium ions, and buffer. Function: Taq DNA-polymerase: Enzyme is heat-resistant as it can withstand the highest temperature during the PCR process. deoxynucleotides (dNTPs): Nucleotides that are used in the replication of new strands. Oligonucleotide primers: Pieces of DNA complementary to the template that tell DNA polymerase exactly where to start making copies. Magnesium ions: A cofactor (catalyst) required by DNA polymerase to create the DNA chain. Salt buffer: Provides the optimum ionic environment and pH for the PCR reaction.
What are the function of buffer?
the function of a buffer is to maintain the pH of the sample.
Function of a frame buffer in computers?
function of a frame buffer in computer?
Why 10x buffer use in pcr?
It provides a suitable chemical environment for optimum activity and stability of the DNA polymerase.
What is the function of MOPS buffer RNA isolation?
It act as a buffer in Northern blotting.
What is the function of TNE buffer?
We use it for isolation of proteins from yeast cells as a lysis buffer
What is the function of PBS buffer?
The main function of PBS (phosphate buffer saline) is to maintain the pH of the solution. by Himanshu Goel Jaypee University of Information Technology
What is the function of formamide loading buffer?
To denature DNA
What is the function of tris and EDTA buffer?
solubilize DNA
Why using phosphate buffer and magnesium sulphate and calcium chloride and ferric chloride for dilution water in BOD5 test?
magnesium sulphate, calcium chloride and ferric chloride are added to provided optimum environment and phosphate buffer is added to slow down the rate of death of microbes.
