0
It provides a suitable chemical environment for optimum activity and stability of the DNA polymerase.
Wiki User
∙ 13y ago
Add your answer:
Earn +20 pts
In this procedure a 10x buffer is used if you have a 5x buffer and you want a total volume of 200ul what dilution would you perform to achieve the desired 1x concentration of buffer?
10x to 1x is a 1:10 dilution Therefore, add 1 part buffer, 9 parts DI-water If 100uL is 10uL (1 part buffer) and 90uL (9 parts DI-water) Then, 200ul (100 x 2) is 20uL (1 part buffer) and 180uL (9 parts DI-water)
What is the difference between simplex and multiplex pcr?
what is the difference between PCR simplex and multiplex
What are the differences between conventional pcr andreal time pcr?
PCR allows amplification of DNA for a specific gene, after too many cycles of PCR the result will reach saturation, basically meaning all of the DNA has been amplified. Conventional PCR will basically tell you whether or not a gene is expressed in your sample. This can be done semi-quantitavely if the PCR is performed for a low number of cycles, ie it will tell you whether one sample expresses more of your gene of interest than another sample. The results are seen by separating the PCR products by agarose gel/ethidium bromide electrophoresis. Real-time PCR will record exactly what cycle of PCR a detectable level of amplified product became detectable, giving a far more accurately quantifiable estimation of gene expression.
What does the 10x on the medium power objective of a microscope mean?
The power of a microscope magnification is the eye piece power times the objective lens so 10X eye piece times 10X objective is 100 power Common eyepieces are 10x 15x, 20X. The limit is about 2000X in an excellent unit. Average practical use is about 1000X to 1400X In expensive scopes the higher power objective lenes as 100X are made from oil not glass.
Effect of Diluting a buffer?
The buffer capacity increases as the concentration of the buffer solution increases and is a maximum when the pH is equal to the same value as the pKa of the weak acid in the buffer. A buffer solution is a good buffer in the pH range that is + or - 1 pH unit of the pKa. Beyond that, buffering capacity is minimal.
What is the purpose of the buffer in PCR?
The purpose of the buffer in PCR, I assume you talking about the 5 or 10 times PCR buffer that is provided with enzyme. Buffer is needed to give the correct pH and pottasium ion concentration for the DNA polymerase enzyme (usually DNA polymerase from Thermus aquaticus) to function.
What is the function of tris hcl in PCR buffer?
IT act as a buffering agent to maintain the PH of a PCR
How do you convert 1x buffer to 10x buffer?
The stocks are commonly labeled as X factors such as 10X, 5X, 100X etc. X-factor indicates that the solution is concentrated and must be diluted usually with water to 1X concentration for use. For eg: - A 100X concentrated solution should be diluted to 100 fold. to convert 1X to 10X take one ml of 1x buffer in a measuring cylinder and dilute it to make it 10 ml. its now 10x buffer.
What is the use of dNTP?
The use of dNTP is PCR and multiplex PCR
The pH of 10x PBS buffer?
PBS pH usually ranges between 7.2 and 7.6.
In this procedure a 10x buffer is used if you have a 5x buffer and you want a total volume of 200ul what dilution would you perform to achieve the desired 1x concentration of buffer?
10x to 1x is a 1:10 dilution Therefore, add 1 part buffer, 9 parts DI-water If 100uL is 10uL (1 part buffer) and 90uL (9 parts DI-water) Then, 200ul (100 x 2) is 20uL (1 part buffer) and 180uL (9 parts DI-water)
Why do you use a negative control in PCR?
to check is there any contamination in pcr products
What is the Buffer purpose in PCR?
buffers in all biochemical reactions mediate pH and keep it within ranges where the reactions will happen. If pH gets too high or too low, the catalytic reactions of PCR will not take place.
Use of water in pcr?
enzyme cofactor
What are the different types of polymerase chain reaction techniques?
types of pcr: AFLP -PCR. Allele-specific PCR. Alu-PCR. Assembly -PCR. Assemetric -PCR. Colony -PCR. Helicase dependent amplification. Hot start pCR. Inverse -PCR. Insitu -pCR. ISSR-PCR. RT-PCR(REVERSE TARNSCRIPTASE). REAL TIME -PCR
What is a Taqman Real Time PCR?
One can use a PCR to amplify and quantify a certain DNA molecule. A TaqMan real-time PCR is a certain type of PCR which uses the TaqMan method to increase its specificity.
What is the use of maintaining a certain pH in pcr?
PCR is an enzymatically guided process. In optimum pH the enzyme will work best.
