Actually the problem with the Human polymerase is the sensitivity to temperature if we talk about PCR. That is the reason why we use Taq DNA polymerase which is thermostable where as use of human polymerase may result in loss of its function due to high temperature.
during the complexometric titration using edta it is very necessary to maintain the ph of the solution near about 10 so we use ammonium chloride buffer if we will not use this buffer dring the titration ph of sol. will ho lower side
You use a buffer when making agarose gels so that when the gel is used for electrophoresis, the gel is able to conduct electricity. The buffer contains ions from the buffer salts that will facilitate conduction. that was good
Yes you need to know what molarity of the phosphate buffer you need to make and what voluime, then you can use Henderson-Hasselbalch equation. or simply use the phophate buffer calculator http://home.fuse.net/clymer/buffers/phos2.html
PHT APP: buffer solutions are used frequently in pharmaceutical practice,particularly in the formulaton of opthalmic solutions
To effectively solve buffer problems, one should first identify the components of the buffer solution (acid and conjugate base), calculate the initial concentrations, and use the Henderson-Hasselbalch equation to determine the pH. Adjusting the ratio of acid to conjugate base or adding more buffer solution can help maintain a stable pH.
the common collector can use as voltage buffer
To effectively write buffer equations, one should first identify the components of the buffer solution, which typically include a weak acid and its conjugate base, or a weak base and its conjugate acid. Next, use the Henderson-Hasselbalch equation to calculate the pH of the buffer solution. Finally, write the buffer equation by balancing the chemical reaction between the weak acid/base and its conjugate base/acid.
To determine the pH of a buffer solution, you can use a pH meter or pH indicator strips. Alternatively, you can calculate the pH using the Henderson-Hasselbalch equation, which takes into account the concentration of the acid and its conjugate base in the buffer solution.
buffer circuits helpful in overcoming the impedance matching problem.for example if we want to send a signal from one devise to other ,if there is no impedance matching between this two then signal is not transfered.now if we use buffer in between these two devises then the buffer without changing the signal shape or value it simply transffers the signal
To maintain constant the pH of a solution.
It is unsafe. In order to use gets() safely, you need to know how many characters you will be reading to ensure your character buffer is large enough: char buffer[10]; while (gets (buffer) != 0) { ...process buffer... } The above code has undefined behaviour when the number of characters read is 10 or more (you need one character for the null-terminator). This is because the character buffer, str, decays to a pointer (referencing &str[0]) and the function, gets(), cannot determine the number of characters in a buffer by its pointer alone. The gets() function was dropped from the C standard in 2011, however some implementations still include it. To avoid the warning, use the fgets() function instead. This allows you to specify the length of your buffer and (when used correctly) prevents buffer overflow. char buffer[10]; while (fgets (buffer, 10, stdin) != 0) { ...process buffer... }
Decide on the concentration of the buffer, use 1L to be simple PH for your buffer should be within one pH unit from the pKa of the acid/conjugate base use Henderson Hasselbalch Equation pH = pKa + log ([Base]/[Acid]) For a 1 M buffer [Acid] + [Base] = 1
You didn't say which meaning you want to use for this word. A buffer can be a barrier which lessens or absorbs a shock. The airbag acts as a buffer in case of an accident. She was the buffer between them, and kept the argument from getting out of hand. Buffer can also mean a chemical substance which minimizes the acidity of a substance. I need a bottle of buffered aspirin; the buffer keeps it from upsetting your stomach. Buffer can also be a verb meaning to do either of the above. The neutral zone between the two countries buffered their rivalry. That substance buffers the chemicals.
Increase the concentration of salt and acid or base. If you are not suppose to increase concentration use more volume of buffer.
The stocks are commonly labeled as X factors such as 10X, 5X, 100X etc. X-factor indicates that the solution is concentrated and must be diluted usually with water to 1X concentration for use. For eg: - A 100X concentrated solution should be diluted to 100 fold. to convert 1X to 10X take one ml of 1x buffer in a measuring cylinder and dilute it to make it 10 ml. its now 10x buffer.
To make a borate buffer, mix boric acid with sodium hydroxide or sodium borate in water. Adjust the pH of the buffer to your desired range by adding more acid or base. Remember to use a pH meter to accurately measure the pH of the buffer.