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Why are living unstained bacterial preparations more difficult to observe?
Wiki User
∙ 11y ago
Living unstained bacterial preparation is more difficult to see than stained preparations because unstained bacteria is transparent . Living bacteria is also very quick and would be hard to see and track without stain.
Wiki User
∙ 9y ago
Wiki User
∙ 13y agoAble to view light sensitive organisms. Doesn't involve heat fixing or staining which usually kill and/or distort organisms. Specimens that lack contrast with their background can be seen (spirochetes that causes syphilis). Permits examination of internal structures.
Wiki User
∙ 12y agoThe importance is to see the bacteria in their natural size and shape. When a bacteria is stained it may be altered in both size and shape by heat fixation and chemicals.
Wiki User
∙ 11y agobecause bacteria is hazardous,
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Explain why crystal violet would not be useful in the negative stain technique?
Negative staining techniques are designed to stain everything BUT the bacterial cells. This allows us to see the cells unstained and helps us observe their morphology (how the cells are shaped and how they group together). The cells have an overall negative charge on their surface, so they naturally attract positive charges. Crystal Violet carries a positive charge, so it would adhere to the surfaces of the cells thus staining the cells (which is not what you want in a negative stain!)
Why are unstained bacteria more difficult to observe than stained bacteria under the microscope?
Three reasons: 1. Bacteria, like most life, are largely composed of water. Water is clear. 2. Bacteria are very small; their size, generally, is only barely within the resolution range of typical brightfield scopes 3. Bacteria are small, and so reflect very little light. A transmission scope depends on reflection to form images.
Can the hanging drop method be used to examine other organisms?
Hangng drop method is used for bacterial examination of fresh preparations-- meaning those which are alive so you can observe their motility, its usually true motility or the brownian movement. This is accomplished by having a drop of liquid preparation into a concave slide with its circular sides filled with vaseline to prevent evaporation. then a cover slip is applied. I dunno what you mean by your question by " other organisms" obviously you can only apply it to bacteria. examples like Staphylococcus aureus, G. tetragena or P. vulgaris.
What range of bacterial morphologies did you observe in the tooth and gum scraping?
The range of bacterial morphologies observed in tooth and gum scrapings are usually anaerobic and cocci. Cocci bacterium have a sphere shape. Cocci are able to live on their own, or form clusters. Anaerobic bacteria grow in the human digestive tract and require little to no oxygen.
How do you Observe these life forms?
Observe these life forms
Why are living unstained bacterial preparation more difficult to observe microscopically than stained preparations?
Staining adds contrast making the bacteria easier to see.
Which characteristic of a landscape is most difficult to observe?
rate of erosion
What planet do scientists not observe as much as all the other planets?
Scientists do not observe Mercury as often because it is difficult to do so.
Which soil properties can you observe?
You can observe if the soil is clay, sand, or full of organic matter by the color. You can observe if the soil is compacted by how difficult it is to dig. You can observe if it has worms which is good because worms provide aeration and fertilizer. You can observe how wet the soil is.
How do you examine live bacteria microscopically?
You observe bacterial smear using oil immersion lens with a magnification of 100x.
Which of the life processes is the most difficult to actually observe in a living animal?
Reproduction
A substance always has properties even though they are difficult to observe?
chemical properties
Explain why crystal violet would not be useful in the negative stain technique?
Negative staining techniques are designed to stain everything BUT the bacterial cells. This allows us to see the cells unstained and helps us observe their morphology (how the cells are shaped and how they group together). The cells have an overall negative charge on their surface, so they naturally attract positive charges. Crystal Violet carries a positive charge, so it would adhere to the surfaces of the cells thus staining the cells (which is not what you want in a negative stain!)
How is Pluto difficult to find?
Pluto is difficult to observe from Earth because it is about 3.66 billion miles away, and only appears as a faint point of light.
Why would it be difficult for a space probe to observe eruptions in progress on moons and planets?
because of your mom
Why are leopards so difficult to observe in the wild?
one can not watch one that moves fast. endangered also.
What three technology that humans use to observe object that difficult to visit?
Telescopes, spectroscopes, space probes.
