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Gram staining is the initial procedure, crutial in determining whether the unknown organism is Gram positive or Gram negative. Once the type is concluded, it is earsier to chose which type of antibiotic will be best to kill the bacterial growth.
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∙ 16y ago
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∙ 9y agoIt is one of first steps which will help you eliminate many genus types of bacteria. In 1884 the Dadnish bacteriologist Christina Gram developed a staining technique that separates bacteria into two groups: Gram-positive and Gram-negative. The procedure is based on the ability of microorganisms to retain the purple color of crystal violet during decolorization with alcohol. Gram-neg. bacteria are decolorized by the alcohol, losing the purple color of crystal violet. Gram-pos. bacteria are nor decolorized and remain purple. After decolorization, safranin, a red counterstain is used to impoart a pink color to the decolorization gram-negative organisms.
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∙ 14y agoif you don't establish gram staining reaction and morphology in the identification of the bacterial isolate, you won't know the gram staining reaction properties and morphology identities of the bacterial isolate.
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∙ 11y agoGram staining indicates what type of cell membrane the cells have (Gram possitive or negative). It can be important in choosing a medication to treat an infection or as a beginning step in identifying a certain type of bacteria.
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∙ 12y agoI guess to identify if it is negative or positive
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∙ 10y agoto distinguish among the many kind of bacteria
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Why was koch's recommemdation for fixing and staining important for microbiology?
Koch's recommendation for fixing and staining is important for microbiology even though all bacteria can be seen under the microscope, because staining and fixing is needed in order to see inside of a transparent bacteria.
Which step is most crucial or most likely to cause poor results in the gram stain?
Gram staining is a type of differential staining in which two types of bacteria are differentiated on the basis of their cell wall either gram positive or gram negative although all the steps in gram staining are crucial, the most important step the most crucial step in the performance of the Gram staining procedure is the decolorization step which is the Acid-Alcohol (3% HCl and 95% Ethanol) and must be timed correctly; the crystal violet stain will be removed from both Gram-positive and negative cells if the decolorizing agent is left on too long (a matter of seconds).
What bacterial staining technique is it when a basic dye is utilized to stain bacterial cells?
A basic dye used in gram staining is crystal violet.
Are there endospores in pseudomonas aeruginosa gram staining?
yes
What are four possible results of a gram stain?
The four possible results after Gram staining are Gram positive, Gram negative, Gram variable and Gram in-determinant.
What type of gram is H1N1 bacteria gram-positive or gram-negative?
neither...it is a virus, and gram-staining, a microbiology tool, is not used to help identify or classify a virus
Why is gram staining important?
It tells the microbiologist/clinician facts about the cell wall construction of the bacterium. This then indicates which antibiotics to prescribe if you are trying to get rid of a bacterial infection.
Why is it important to follow the gram staining procedure?
This is simply important in order to have accurate staining results. If this is not followed, the process of the staining will result to false positives or false negatives.
Why was koch's recommemdation for fixing and staining important for microbiology?
Koch's recommendation for fixing and staining is important for microbiology even though all bacteria can be seen under the microscope, because staining and fixing is needed in order to see inside of a transparent bacteria.
What are the names of special stains in microbiology?
In microbiology the concept of staining is very important because it highlights the structures of microorganisms allowing them to be seen under a microscope (because ordinarily the microorganisms are somewhat transparent making them difficult to see). In the case of some bacteria, many have specific surface structures such as capsules and flagella as well as internal components such as endospores. To specifically enhance these structures, a special stain may be used. An example of this is using negative staining techniques to see capsules, or using the Ziehl-Neelsen technique to see endospores.
Why to gram stain bacteria?
gram staining is a biochemical method of identifying bacteria in a more specific way.Thus it is important to differentiate gram positive and gram negative bacteria.
Is gram staining related to flagella?
No. It is a staining on the cell itself.
Who developed Gram staining?
Gram staining was devised by Hans Christian Gram of Denmark in the 1800s. (1853-1938)
Why gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Why is Gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Did you stain the bacterial cell wall or the cell cytoplasm?
Yes, it is. Using the Gram staining method, the wall can be seen.
Which step is most crucial or most likely to cause poor results in the gram stain?
Gram staining is a type of differential staining in which two types of bacteria are differentiated on the basis of their cell wall either gram positive or gram negative although all the steps in gram staining are crucial, the most important step the most crucial step in the performance of the Gram staining procedure is the decolorization step which is the Acid-Alcohol (3% HCl and 95% Ethanol) and must be timed correctly; the crystal violet stain will be removed from both Gram-positive and negative cells if the decolorizing agent is left on too long (a matter of seconds).
