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What is difference between isocratic and gradient hplc?
in isocretic system we use only single mobile phase but in gredent system we use two or more mobile phases.
What does it means if a substance moves down its concentration gradient?
If a substance moves down its concentration gradient, it means that it is moving from an area where it has a high concentration to an area where it has a low concentration. This is known as diffusion.
ATP synthase works by means of?
a proton gradient across the inner mitochondrial membrane
What is the gradient on mercury?
The answer depends on the gradient of WHAT!
For diffusion to occur there must be?
For diffusion to occur there needs to be a concentration gradient. Which means that the concentrations on the two sides must be different. In diffusion the particles flow from high concentration to low concentration.
Why retention time vary during isocratic analysis?
Using isocratic retention parameters, the gradient elution retention time for several proteins has been calculated. The gradient retention time calculation is based on fitting the isocratic retention data to an equation of the form: log k′ = m log (1/[Ca2+]) + log K and on applying well-established principles of gradient elution. A good correlation between the observed and calculated retention times for several test proteins was obtained at various total gradient times and column flow-rates.Conversely, isocratic retention parameters characterizing protein retention can be calculated from gradient elution retention data. However, even with retention data of high quality, small errors are amplified by the log-log nature of the ion-exchange isocratic retention model employed.Based on the close correlation between predicted and observed gradient retention times, no evidence for protein denaturation resulting from immobilization of the protein at high initial k′ values at or near the column inlet was observed.
What is difference between isocratic and gradient hplc?
in isocretic system we use only single mobile phase but in gredent system we use two or more mobile phases.
What happen you changed the percentage composition of mobile phase in HP LC?
if you are doing isocratic elution nothing will change at all but in case pf gradient analysis elution order may change.
What is gradient force?
A force gradient means the force is different in one location than it is in another. It is simply not constant but a function of position.
What does the River flow velocity depends on?
the gradient and how much friction there was. The gradient means how steep the land the river is on so if it is very steep them the velocity will be higher.
What does the slope of a veloctiy time graphy represent?
The slope represents a change in velocity, or acceleration. The acceleration is the gradient (steepness) of the line. A larger gradient means faster acceleration. if the gradient points down and is negative then it represents deceleration.
What does steepening mean?
It means to make steeper or to increase the absolute value of the gradient.
A high concentration gradient means that the concentration of a substance is?
It means one side have high concentration.Other side has lower.
What does gradient mean in physics?
Gradient means the vector derivative (Del) of a scalar quantity, e.g. for the Gravity Potenttial/scalar energy, Del-mGm/r= mw2R.
How do you find the gradient of a line using y equals 7x plus 2?
When the equation is given in the standard form: y = mx + c, the gradient is m. So here, y = 7x + 2 means m, the gradient, is 7.
What does it means if a substance moves down its concentration gradient?
If a substance moves down its concentration gradient, it means that it is moving from an area where it has a high concentration to an area where it has a low concentration. This is known as diffusion.
ATP synthase works by means of?
a proton gradient across the inner mitochondrial membrane
