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The opacity determines how transparent a layer in Photoshop is. Setting the opacity at 0% will make the layer completely invisible, revealing any layers underneath it. Setting the opacity at 50% will make the layer half visible, making both the layer, and the layers under it equally visible. Setting the opacity to 100% will make the layer completely visible, anything under it will be completely covered.

This can be useful when changing eye colors, as if the color you've changed the eye to looks too unrealistic, you can lower the opacity to allow the original eye color and detail to show through, which can make for a more realistic effect.

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