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The indicator is bromothymol blue.
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What does the citrate metabolic test test for?
it tests the ability of a bacteria to use citrate as its sole carbon source. if its positive, it will raise the pH and there will be no acid in the end product.
What substance is used to determine the presence of an acid or base?
by using an indicator like litmus paper!!!
Why is water used as the universal indicator?
Water is NOT Universal Indicator. Water is often referred to as a Universal Solvent as a large number of substances are soluble in it. Universal Indicator is a chemical agent that can be used to test the pH of a liquid.
Why was universal indicator paper used to test the pH rather than placing universal indicator solution into the mixture?
Use of paper avoid the contamination of the sample.
Why is the citrate test carried out on an agar slope rather than a broth?
Background:Simmons' Citrate Agar is a defined, selective medium that tests for an organism's ability to use citrate as a sole carbon source and ammonium ions as the sole nitrogen source.The medium contains citrate, ammonium ions, and other inorganic ions needed for growth.It also contains bromothymol blue, a pH indicator. Bromothymol blue is green at pH below 6.9, and then turns blue at a pH of 7.6 or greater.SO:Sometimes, it is possible to detect growth on the citrate slant without the accompanying color change to blue. This is most likely due to insufficient incubation. Either a combination of blue color and growth or growth alone without the blue color should be scored as a positive for the citrate use test.
Citrate utilization test?
The test indicates if an organism utilizes Citrate as it's only source of Carbon. Positive results will show growth on slant as well as changing the medium from green to blue (ph indicator)
What is simmons citrate agar?
Simmons citrate agar is a differential agar used to determine if a sample bacteria can utilize citrate as its only carbon source. The agar is initially a green color due to the bromo thymol blue pH indicator in it. If a bacteria uses the citrate, the by-products are ammonia and ammonium hydroxide both of which will alkalize the agar and increase the pH to the point of changing the indicator's color to blue, so the whole agar turns from green to blue.
What is universal indicator state the purpose for which this indicator is used?
Universal Indicator is used to test for the acidity of a solution, because the universal indicator has a wider range of pH that it can explain why a substance is acidic or basic.
What does the citrate metabolic test test for?
it tests the ability of a bacteria to use citrate as its sole carbon source. if its positive, it will raise the pH and there will be no acid in the end product.
In a pH test how much wide range indicator to use for 5 ml?
A pH paper can be used also for a small sample.
How do you test pH of strong acid?
universal indicator
What are three safe methods to test for an acid?
1. Test with a pH paper. 2. Test with a liquid or solid pH indicator. 3. Measure the pH with a pH-meter.
What substance is used to determine the presence of an acid or base?
by using an indicator like litmus paper!!!
Why is water used as the universal indicator?
Water is NOT Universal Indicator. Water is often referred to as a Universal Solvent as a large number of substances are soluble in it. Universal Indicator is a chemical agent that can be used to test the pH of a liquid.
What is the principle of bromocresol purple in albumin test?
pH indicator
Why was universal indicator paper used to test the pH rather than placing universal indicator solution into the mixture?
Use of paper avoid the contamination of the sample.
Why is the citrate test carried out on an agar slope rather than a broth?
Background:Simmons' Citrate Agar is a defined, selective medium that tests for an organism's ability to use citrate as a sole carbon source and ammonium ions as the sole nitrogen source.The medium contains citrate, ammonium ions, and other inorganic ions needed for growth.It also contains bromothymol blue, a pH indicator. Bromothymol blue is green at pH below 6.9, and then turns blue at a pH of 7.6 or greater.SO:Sometimes, it is possible to detect growth on the citrate slant without the accompanying color change to blue. This is most likely due to insufficient incubation. Either a combination of blue color and growth or growth alone without the blue color should be scored as a positive for the citrate use test.
