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What are the results for klebsiella pneumoniae in the citrate utilization test?
Klebsiella pneumoniae is typically citrate negative, meaning it does not utilize citrate as a carbon source in the citrate utilization test. This bacterium lacks the enzyme citrate permease needed for citrate utilization.
What is the positive organism for citrate utilization test?
The positive organism for citrate utilization test is usually Escherichia coli. When this bacterium is able to grow on a citrate-containing medium, it will produce alkaline byproducts that change the pH of the medium, turning it from green to blue. This color change indicates a positive result for citrate utilization.
Does serratia test negative for citrate?
Yes, Serratia marcescens typically tests negative for citrate utilization in citrate utilization tests. This bacterium lacks the enzyme citrate-permease needed to import citrate into the cell, resulting in a negative citrate test.
What does the citrate utilization test determine?
The citrate utilization test determines the ability of an organism to use citrate as its sole carbon source for growth. It is commonly used to differentiate between members of the Enterobacteriaceae family based on their ability to utilize citrate.
Is Morganella morganii positive for a citrate test?
Yes, Morganella morganii is positive for citrate utilization. This bacterium can use citrate as a carbon source for growth and will produce alkaline byproducts, causing a color change in the citrate test medium from green to blue.
Is kocuria rosea citrate positive?
Yes, Kocuria rosea is typically citrate positive, meaning it can utilize citrate as a carbon source for energy production. This ability is often tested in the laboratory using a citrate utilization test.
Why does a inoculate citrate slant and incubate for 48 hours change from green to blue?
The change from green to blue in an inoculated citrate slant after incubation indicates the utilization of citrate by the bacteria, causing a pH increase in the medium. This change is associated with the conversion of citrate to alkaline compounds and the subsequent utilization of citrate as a carbon source for growth. This test is commonly used to differentiate between organisms that can utilize citrate and those that cannot.
What does the citrate metabolic test test for?
The citrate utilization test assesses an organism's ability to utilize citrate as a carbon source for growth. It is often used to differentiate between different species of bacteria, such as in differentiating between Citrobacter freundii and Salmonella species. If an organism can utilize citrate, it will demonstrate growth and turn the medium alkaline.
Is Shigella Positive for a citrate test?
Based on my own test, Shigella flexneri tests negative for the citrate test.
What is Procedure of assay test of sodium acid citrate?
assay test of sodium acid citrate
What IMVic results would indicate the presence of fecal coliforms?
The IMViC test results that would indicate the presence of fecal coliforms are negative for indole production, positive for methyl red test, negative for Voges-Proskauer test, and positive for citrate utilization.
Why is the citrate test carried out on an agar slope rather than a broth?
Background:Simmons' Citrate Agar is a defined, selective medium that tests for an organism's ability to use citrate as a sole carbon source and ammonium ions as the sole nitrogen source.The medium contains citrate, ammonium ions, and other inorganic ions needed for growth.It also contains bromothymol blue, a pH indicator. Bromothymol blue is green at pH below 6.9, and then turns blue at a pH of 7.6 or greater.SO:Sometimes, it is possible to detect growth on the citrate slant without the accompanying color change to blue. This is most likely due to insufficient incubation. Either a combination of blue color and growth or growth alone without the blue color should be scored as a positive for the citrate use test.
