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How can you differentiate Serratia marcescens and Shigella flexneri as an unknown?
A simple test you can perform is the Citrate test, Serratia marcensens is Citrate positive while Shigella flexneriis citrate negative.Also Serratia Marcensens is motile and Shigella flexneri is non motile if you have already done a Hydrogen Sulfide test you would have been able to already determine between the two.Also Serratia marcensens is VP positive while Shigella flexneri is negative.Serratia marcensens is lipase positive and Shigella flexneri is lipase negativeAnother distinguishing characteristic is that Serratia marcensens grows a distinct red color pigmentation the Shigella flexneri does not have.(These are all according to Bergeys Manual of Systematic bacteriology)
What is the positive organism for citrate utilization test?
The positive organism for citrate utilization test is usually Escherichia coli. When this bacterium is able to grow on a citrate-containing medium, it will produce alkaline byproducts that change the pH of the medium, turning it from green to blue. This color change indicates a positive result for citrate utilization.
Bacillus cereus have a positive reaction for VP and citrate?
The B.cereus has a positive citrate test and a negative Voges Proskauer.
Is Morganella morganii positive for a citrate test?
Yes, Morganella morganii is positive for citrate utilization. This bacterium can use citrate as a carbon source for growth and will produce alkaline byproducts, causing a color change in the citrate test medium from green to blue.
What are the results for klebsiella pneumoniae in the citrate utilization test?
Klebsiella pneumoniae is typically citrate negative, meaning it does not utilize citrate as a carbon source in the citrate utilization test. This bacterium lacks the enzyme citrate permease needed for citrate utilization.
Does serratia test negative for citrate?
Yes, Serratia marcescens typically tests negative for citrate utilization in citrate utilization tests. This bacterium lacks the enzyme citrate-permease needed to import citrate into the cell, resulting in a negative citrate test.
What is the result of citrate test for bacillus subtilis?
blue/positive
Is kocuria rosea citrate positive?
Yes, Kocuria rosea is typically citrate positive, meaning it can utilize citrate as a carbon source for energy production. This ability is often tested in the laboratory using a citrate utilization test.
Citrate test result for Pseudomonas aeruginosa?
Pseudomonas aeruginosa typically demonstrates a negative result on the citrate test, as it is unable to utilize citrate as its sole carbon source for growth. This bacterium lacks the enzyme citrate-permease needed to transport citrate into the cell for metabolism, leading to a lack of growth on citrate agar and a lack of color change from green to blue.
What is the result of citrate test for bacillus licheniformis?
The result is the initial green color of the Simmons Citrate Agar turning blue; generating a positive indication that the organism does indeed utilize citrate as the sole source of carbon and energy.
Is bacillus cereus positive for methyl red?
Most bacteria in Tribe V are able to utilize Citrate, with the exception of Hafnia and Pantoea (+/-). The 2 most common Klebsiella (oxytoca and pneumoniae), can utilize citrate, so should be citrate positive.
Is shigella positive for nitrate?
yes
