yes
Shigella species are generally indole negative, except for Shigella flexneri, which can be indole positive depending on the specific strain. In laboratory identification, indole testing is used to differentiate between various enteric bacteria. Therefore, while most Shigella species do not produce indole, Shigella flexneri may show positive results under certain conditions.
Based on my own test, Shigella flexneri tests negative for the citrate test.
No, Shigella dysentery is not acid-fast positive. Shigella is a genus of bacteria that are Gram-negative and do not retain the stain used in acid-fast bacilli (AFB) staining. Acid-fast positivity is characteristic of mycobacteria, such as Mycobacterium tuberculosis, not Shigella. Therefore, Shigella dysentery would not be identified as acid-fast in laboratory tests.
Shigella is catalase positive. This means that it produces the enzyme catalase, which breaks down hydrogen peroxide into water and oxygen. This characteristic can be used to help differentiate Shigella from other enteric bacteria in laboratory settings.
urease - negative ONPG - positive Lysine - positive
Yes, almost Enterobacteriae are positive for nitrate reduction.
When a gram stain is done on Shigella flexneri, the cells are light pink. This means that they are gram negative.
A simple test you can perform is the Citrate test, Serratia marcensens is Citrate positive while Shigella flexneriis citrate negative.Also Serratia Marcensens is motile and Shigella flexneri is non motile if you have already done a Hydrogen Sulfide test you would have been able to already determine between the two.Also Serratia marcensens is VP positive while Shigella flexneri is negative.Serratia marcensens is lipase positive and Shigella flexneri is lipase negativeAnother distinguishing characteristic is that Serratia marcensens grows a distinct red color pigmentation the Shigella flexneri does not have.(These are all according to Bergeys Manual of Systematic bacteriology)
shigella sp or shigella spp = shigella species
Sodium will be positive. Check your periodic table; all those elements on the left will always be positive ions.
When testing silver nitrate, a whitish color will yield positive results. If the test is negative, the color will turn clear and it will remain transparent.
To differentiate Shigella flexneri from Yersinia pseudotuberculosis, a key lab test is the urease test. Yersinia pseudotuberculosis is urease positive, while Shigella flexneri is urease negative. Additionally, biochemical tests such as the fermentation of lactose can help, as Shigella flexneri does not ferment lactose, whereas Yersinia may show variable lactose fermentation.