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How pH of culture be influenced by urea hydrolysis?
It will cause the culture to become more basic...an increase in pH
What is the function of urease?
Hydrolysis of urea to carbon dioxide and ammonia is catalyzed by the enzyme urease.
What is the difference between acidic and alkaline hydrolysis?
Acidic hydrolysis: hydrolysis in an acid solution (pH under 7) Basic hydrolysis: hydrolysis in a basic solution (pH above 7)
What is the pH of urea?
Under 5,5.
What is the pH indicator in nutrient agar?
None
How pH of culture be influenced by urea hydrolysis?
It will cause the culture to become more basic...an increase in pH
What is the function of urease?
Hydrolysis of urea to carbon dioxide and ammonia is catalyzed by the enzyme urease.
What is the difference between acidic and alkaline hydrolysis?
Acidic hydrolysis: hydrolysis in an acid solution (pH under 7) Basic hydrolysis: hydrolysis in a basic solution (pH above 7)
Can urea neutralise pH water?
Urea water solution is neutral.
What is an arginase?
An arginase is an enzyme which catalyzes the hydrolysis of arginine to form urea and ornithine.
The major chemical digestion that occurs in the adult stomach is a. hydrolysis of fats by gastric lipase b. conversion of ammonia to urea c. hydrolysis of sucrose by sucrase d. hydrolysis of proteins?
D. hydrolysis of proteins
What is the pH of urea?
Under 5,5.
What does a positive test result for urea hydrolysis look like?
Bright pinkish-red.
What is the end product of arginine hydrolysis?
The end products of the hydrolysis of Arginine are Orthinine and urea, several enzymes catalyse this reaction the easiest to remember is arginase.
What is the pH indicator in nutrient agar?
None
Explain how the urease test is useful for identifying members of the genus proteus?
Genus proteus is know for their fast hydrolysis of urea into ammonia. In presence of proteus the agar slant quickly turns pink (positve) while known proteus would be yellowish. The pink color is a sign of alkaline presence and the increase of pH (7-8.5) and phenol red is the pH color indicator. http://www.microbelibrary.org/Laboratory%20Diagnostics/details.asp?id=457&Lang=
Which products of fat hydrolysis would cause a change in pH of the whole milk sample?
The pH of a milk sample will go down due to carboylate anions. The hydrolysis is performed with triacylglycerols to glycerol.
