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As the name suggests there is an antigen - antibody reaction between the antigen (Hormone) of interest and its specific antibodies raised from laboratory animals. The quantum of antigen-antibody binding is monitored using a radiolabelled antigen or antibody. In brief the RIA kit contains test tubes which have been coated with the antibody. To these tubes, standards or samples are added (usually 25 to 200 ul volume) along with the tracer, The contents are incubated for 1 - 2 hours and the tubes are emptied by decanting the contents or aspirating. The tubes are washed with the wash solution provided and are counted in a gamma counter. The counts obtained for various known standards used in the assay are utilised for generating a dose - response curve and the unknown concentration in serum samples are extrapolated from the data. \
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