The Rothera nitroprusside test looks for ketone bodies in urine. It is done by taking 5 mL of urine and adding ten drops of concentrated ammonia water and solid ammonium sulfate. The sample is then analyzed after 15 minutes. If the sample turns purple, then that means there is acetoacetic acid in the urine.
on adding ammonium sulphate the proteins get precipitated and settles down. so the ketone bodies floats on the surface. the surface of these ketone bodies(acetone and acetoacetic acid) react with sodium nitroprusside and strong ammonia to give purple coloured complex.
pH indicator
detects deoxy sugar
The principle of the heat coagulation test for proteins involves the reaction of amino acids and Ninhydrin. When these two react together under high temperatures, fingerprints can be seen.
the iodine will react with carbohydrate to give different color .
hydrolysis of pyrophosphate to phosphate forming yellow ppt.
same principle for latex methd
answer it!
This test is used to test for starch prenest in a given solution
complexation
the leave no trace!
Diabetes
the principle of this test are used the method of a qualitative test for the detection of nematode and cestode eggs and coccidia oocysts in the faeces.
pH indicator
Kraut's test for lipids is a test for glycerol. One of the reagents used in Kraut's test for lipids is bismuth subnitrate
detects deoxy sugar
to find out if the substance is acid or akaline
red blodd cell hemolysis