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The Rothera nitroprusside test looks for ketone bodies in urine. It is done by taking 5 mL of urine and adding ten drops of concentrated ammonia water and solid ammonium sulfate. The sample is then analyzed after 15 minutes. If the sample turns purple, then that means there is acetoacetic acid in the urine.
Wiki User
∙ 9y ago
Wiki User
∙ 9y agoon adding ammonium sulphate the proteins get precipitated and settles down. so the ketone bodies floats on the surface. the surface of these ketone bodies(acetone and acetoacetic acid) react with sodium nitroprusside and strong ammonia to give purple coloured complex.
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