Your first step, if the microbe is in a liquid culture is to aseptically streak a nutrient agar plate for isolation. Incubate for 48 hours at 37 degrees C. You should have some good colonal growth after this.
Transfer a small colony, or simply take a sample of the original liquid culture and transfer it to a glass slide.
Perform a Gram stain and view under the microscope at 1000x. Use oil immersion if necessary.
From this you will be able to tell the microbial morphology and gram-stain outcome.
At this point, a series of selective and differential tests will need to be made depending on the outcome of the above. See Bergy's Manual for some more inciteful information.
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