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According to me, we use alcohol because DNA is insoluble in alcohol, it aggregates together, giving a pellet in centrifugal and we can see a precipitated DNA with naked eyes (that we suppose to see in experiment i.e DNA extraction)....
What else can I help you with?
What is the significance of extracting DNA?
What is the significance of DNA?
What is a hypothesis for extracting banana DNA?
Yes dickface
What is the purpose of using detergent in extracting plant DNA?
Detergent denatures (breaks up) proteins, which can then be precipitated and the DNA can be isolated
What is the purpose of using surfactant when extracting DNA?
Surfactants help break down cell membranes and release DNA. They reduce surface tension and facilitate mixing of the sample, allowing for better contact between the solvent and biomolecules. This helps improve the efficiency of DNA extraction.
Name a liquid that DNA is not soluble in?
DNA is not soluble in alcohol.
Why is it easier to isolate DNA from strawberries compared to extracting human DNA?
It is easier to isolate DNA from strawberries compared to extracting human DNA because strawberries have more DNA per cell, making it simpler to extract a larger amount of DNA for analysis. Additionally, the cell walls of strawberries are easier to break down, allowing for easier access to the DNA inside.
Do you extract human DNA the same as animal DNA?
Yes. The DNA is found in the nucleus of every cell. It's simply a case of extracting the DNA from the cell.
What is the purpose of the alcohol in the strawberry DNA extraction lab?
Hmm, I think it's used to wash the DNA from the substance used for extracting the DNA, such as Isopropanol, CLS, NLS, PPS which are used in the process of extraction to the process to precipitate the DNA (using isopropanol). DNA is insoluable in alcohol. Actually the real reason for using alcohol in a DNA extraction is to "precipitate" (NOT RAIN) the DNA, as you may have realized when you did the experiment , the Alcohol step is the last step that brings the DNA to the surface. Finally alcohol can be added so that the DNA can be seen, but first it is crucial to know why the addition of alcohol is such an important step. This step is closely related to a process called precipitation, which is " the process of separating a substance from a solution as a solid" (answers.com, precipitation). Precipitation separates the DNA so that it can no longer remain dissolved and so that it can be seen. DNA is less dense than water and alcohol , so the DNA rises to the alcohol layer. Also the DNA is soluble in water and insoluble in alcohol, so when it is just in the mixture it is quite invisible but once the alcohol is added it condensates by precipitation to the point where it is very visible.
What properties of DNA are responsible for its precipitation in alcohol?
DNA precipitates in alcohol due to its hydrophobic nature and the presence of phosphate groups that interact with alcohol molecules. When DNA is mixed with alcohol, the hydrophobic regions of the DNA interact with the alcohol molecules, causing the DNA to clump together and precipitate out of solution. Additionally, the negatively charged phosphate groups on the DNA backbone can form interactions with the positively charged ions present in alcohol, further promoting DNA precipitation.
Does rum extract contain alcohol?
Yes, rum extract does contain alcohol. It is made by extracting the flavor of rum using alcohol as a solvent.
Can you use spirit for DNA precipitation?
Several DNA isolation protocols recommend the use of either ethyl or isoamyl alcohol for the precipitation step
Why do you use detergent when extracting DNA?
It breaks down the Cell Membrane, making it easier for the DNA to come through. Good Luck :)
