Autoclaving is used to kill all micro-organisms that are in the culture. Autoclaving is usually carried out at 121 deg C with steam under 2 atmospheres of pressure. A clean culture free of all micro-organisms is essential as you only want the organism of intrest to grow.
use a pressure cooker
differential.
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Agar solidifies media, and will remain solid even when placed in an incubator. Few microorganisms are able to metabolize it, so it won't provide an energy source.
In liquid medium one starts by placing one or two loopfuls of the liquid medium directly on the slide. From solid media such as nutrient agar. One starts by placing one or two loopfuls of water on the slide and then using an inoculation loop to disperse the organisms in the water. Bacteria growing on solkid media tend to cling to each other and mus be dispensed suficiently by dilutiion in water.
yes
Autoclaves use high preassure and temperature to kill anything that might infect your media etc.
preparation of media and maintenance of cultures of E.histolytica
The purpose of edge preparation is to achieve the quality characteristics of the workpiece.
Autoclaving media twice may result in nutrient loss and changes in pH and composition which can affect microbial growth. It is generally recommended to prepare fresh media for each use to ensure optimal conditions for microbial growth.
The purpose of food preparation is to get the food ready before you cook it. For example, you want to wash the potatoes before you cook them. That is considered preparation.
by autoclaving at 121°C for 15 minutes
no
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use a pressure cooker
It's purpose was to make preparation for war call for peace and declare independence.
It explains the purpose and meaning of the media piece.