There are various types of nutrient broth, some selective, some not so. They are basically a liquid culture medium to enhance the growth of mircoorganisms from a sample, be it a human clincal sample, food, environmental sample or whatever. After the nutrient broth has been inoculated with the foresaid sample, it is generally incubated for a given length of time to encourage the growth of whatever is in the sample then identified.
In most experiments in which microorganisms are being grown, nutrient broth is used. This is because, nutrient broth (as the term eludes to) has nutrients/food which the microorganisms can feed on and grow. There are different types of nutrients contained in the broth, depending on the type of microorganisms that the researcher is trying to grow or isolate. Sterile, just means that the nutrient broth has undergone a process where by all living cells or organisms that may be in the nutrient broth have been killed. This is done so that when you asceptically innoculate that broth with a microorganism of your choice, there are no other living cells present to contaminate and or grow with your microbes. These unwanted microbes could inhibit or kill the microbes that you are trying to grow.
Hope this helps.
All the Best
S J Kwaramba
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Nutrient broth is a kind or culture medium whcih is generally used used to grow bacteria. It is liquid media and contain beef extracts, peptones ( partially digested proteins) and NaCl.
To provide a growth medium for bacteria.
damn, i forgot what i was going to type
none
Nutrient broth contains beef extract which acts as sources of amino acids, peptides, nucleotides, organic acids, vitamins and mineralsthat peptone broth don't contain? :)
the ampicillin broth and the nutrient broth
Nutrient broth becoming cloudy in appearance is usually a good indication that one or more cultures of bacteria or fungi has grown in the broth. This can be confirmed by viewing a sample of the broth under a simple light microscope, or by streaking a sample onto nutrient agar and incubating it to see if colonies become visible (usually within a few days).
Yes, the turbidity in the unsterilized nutrient is supposed to be incubated, Incubation will give the desired and correct results.
Solid medium
the east extract is the main source of nitrogen in the nutrient broth or agar
Nutrient broth contains beef extract which acts as sources of amino acids, peptides, nucleotides, organic acids, vitamins and mineralsthat peptone broth don't contain? :)
Bacillus subtilis is grown on nutrient agar or in nutrient broth. The preferred medium is M9 with glucose.
nutrient broth + 0.5% particular carbohydrate + indicator
To destroy any competitors for the broth, fungi and bacteria.
NO
the ampicillin broth and the nutrient broth
Nutrient broth is a liquid used to grow bacterial cultures and the amount of sodium chloride is a solution of 0.90% w/v of NaCl.
Nutrient broth becoming cloudy in appearance is usually a good indication that one or more cultures of bacteria or fungi has grown in the broth. This can be confirmed by viewing a sample of the broth under a simple light microscope, or by streaking a sample onto nutrient agar and incubating it to see if colonies become visible (usually within a few days).
no
A nutrient broth is contains substances that are required for the growth of microorganisms such as bacteria. The bacteria obtain their minerals and vitamins from peptone, meat and yeast extract that comprise the broth.
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