The basic purpose of the solvent is to move the pigments up the chromatography paper through capillary action so that the pigments can be separated. The basic importance of chromatography is to separate different solvents by their molar mass. In doing so, one can separate the different pigments within a solution and measure how much of each pigment is present.
to do the dissolving of the solute.
it means that the solvent dissolves the solute.
the more soluble the plant pigment is with the chromatography solvent, the farther it will travel up the chromatography paper
Mobile phase.
Because methanol is a very polar solvent
Chromatography
it means that the solvent dissolves the solute.
the more soluble the plant pigment is with the chromatography solvent, the farther it will travel up the chromatography paper
Column chromatography is generally used as a purification technique: it isolates desired compounds from a mixture.Column chromatography is separated into two categories, depending on how the solvent flows down the column. If the solvent is allowed to flow down the column by gravity, or percolation, it is called gravity column chromatography. If the solvent is forced down the column by positive air pressure, it is called flash chromatography, a "state of the art" method currently used in organic chemistry research laboratories The term "flash chromatography" was coined by Professor W. Clark Still because it can be done in a "flash."
In chromatography you need one stationery phase (paper) and one mobile phase (here solvent, moving by capillary forces)
Mobile phase.
Because methanol is a very polar solvent
Chromatography
water
•Descending chromatography is faster because gravity helps the solvent flow.
Both are techniques of analytical chemistry and use a solvent.
The Rf value cannot be determined since the distance of the solvent may overlay in the paper.
we can say this because both of these are separation technique