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The relationship between the percent volume (not reached by the stain) and the surface area-to-volume ratio would be that the bigger the agar cube size (surface area to volume ratio), the bigger the percent volume.

This is true because resources need to travel a farther distance through the cell ("cover more ground", so to speak) in order to be evenly distributed through the cell.

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Q: What is the relationship between the percent volume not reached by the stain and the surface area-to-volume ratio?
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