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Why plant is incubated with CTAB buffer at 65 degree celsius?
Plant tissues are incubated with CTAB buffer at 65 degrees Celsius to extract high-quality genomic DNA. The CTAB buffer disrupts cell membranes and releases DNA, and the high temperature helps to denature proteins and enzymes that could degrade the DNA. This process allows for efficient isolation of intact DNA for downstream applications like PCR or sequencing.
Tris EDTA buffer in plant DNA isolation?
Chelating agent
What is the plant's source of food?
Minerals in the soil are the food source of the plant.
What are sources of crude drugs?
sources are as follow biological(animal) source plant source mineral source marine source plant tissue culture techniques
Extraction of morphine fromopiumpoppypdf?
extraction ,isolation,characterization of morphine which is obtained from opium poppy plant
Isolation of siderophore producing bacteria from leguminous plants?
yes.we can isolate bacteria from leguminous plant.
What has the author Paul E Sanchez written?
Paul E. Sanchez has written: 'Assessment of solid waste management units at the Waste Isolation Pilot Plant' -- subject(s): Evaluation, Hazardous wastes, Management, Waste Isolation Pilot Plant (N.M.)
What is floral isolation?
Floral isolation is a type of reproductive isolation mechanism in plants where differences in floral structures, such as size, shape, or color, prevent effective pollination between different plant species. This can lead to a reduction in gene flow and eventual speciation.
What is the source of a plant's mass?
The elements (matter) from which the plant is made.
Isolation of genomic DNA from groundnut leaves?
Hello, Its very simple, u can go for the CTAB method , with bit modification, 1) Ensure the youngest possible leaves, very tender enough to crush. 2) Crush the leaf sample in liquid nitrogen. 3) add quickly CTAB buffer to the crushed isolate immediately. 4) the follow Rapid method of isolation.
What exactly is a Xanthomonas isolation Agar medium?
Xanthomonas isolation agar is a specialized medium used to isolate Xanthomonas species, which are plant-pathogenic bacteria. This medium contains specific nutrients and inhibitors to suppress the growth of other bacteria and promote the growth of Xanthomonas. It is used in microbiology laboratories for the selective isolation and identification of these bacteria from plant samples.
What is the difference between animal and plant DNA extraction?
Plant DNA extractionPlant genomic DNA is more difficult to extract because of the plant's cell wall, which is removed by homogenization, or by adding cellulase to degrade the cellulose that makes up the cell wall. Also, the metabolites present in the plant cell may interfere with genomic DNA extraction by contaminating the DNA sample during the precipitation process.Animal DNA ExtractionPeripheral blood leukocytes are a main source of animal genomic DNA, but sample collection is difficult as blood must be withdrawn from the animal. Blood contains a range of compounds like proteins, lipids, white blood cells, red blood cells, platelets, and plasma, which can contaminate the DNA sample. The primary contaminant of animal DNA extracted from blood samples is heme, the non-protein component of hemoglobin.DifferencesThe differences between plant and animal DNA lie in the sequence of bases in the helix. Compounds found in plant cells are absent in animal cells, and DNA base sequences reflect this, as the genomic plant DNA is often larger than animal DNA. These differences affect extraction methods, as it impacts on yield and purity of DNA.
