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It is used as a cryo-protectant. DMSO prevents the formation of ice crystals and prevents cell lysis during thawing.
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What is a cell culture how can A cell culture be useful to biologist?
A cell culture is where cells are grown under controlled conditions.
What is the role of DMEM in cell culture?
It is the medium which contains all the required enriched nutrients essential for the cells to survive in invitro condition.
What is the role of Cloning Host in recombinant DNA technology?
The role of cloning host in RDT is to serve as the host cell under culture, in which the designed gene is subjected to produce recombinant protein.
What you mean by stem cell culture?
The stem cell "culture" is the medium in which the stem cell is able to divide and grow.
Why is DMSO used in PCR?
reduce secondary structure
Why is it important to do endotoxin and hybridoma testing in animal cell culture products like DMSO and media?
In animal cell culture, the requirements for the cell are only those that are beneficial to the cell and which do not cause any harm to the normal physiological activities of the cell. Endotoxins can hamper normal activities of cell and can even lead to cell death. Hybridomas can out-compete the cultured cells for nutrients. Thus, both reduce the activity of the cultured cells. This is why is it important to do testing of cell culture products so that there is no addition of such substances detrimental to the cell.
Why is DMSO used as a control?
Many test compounds (drugs, inhibitors, etc) are not soluble in water and therefore dimethylsulfoxide (DMSO) is used as a solvent instead. The compound dissolved in DMSO is what is used to treat the cell or animal and therefore you must prove that it is the compound, not the DMSO which is causing any results seen. To ensure this, you have a control that contains only DMSO and not the test compound. This is often referred to as a "vehicle control".
What is the role of FBS in mammalian cell culture?
FBS provide amino acids for the cell growth
What is the role of DMSO in Transformation storage Solution?
it serves as an anti-freezing and cryo-protectant for the cells.
What is the role of glucose in cell culture?
Glucose is an essential component and a carbohydrate source that facilitates the growth of cells.
What is the difference betwen DMSO and DMSO-d6 solution?
One difference is that DMSO-d6 is going to cost more. Chemically speaking, the diffference is that the hydrogens in the -d6 variety have been replaced by deuterium.
What is a cell culture and how can a cell culture be useful to biologists?
A cell culture is where cells are grown under controlled conditions.
What is a cell culture how can cell culture be useful to biologists?
A cell culture is where cells are grown under controlled conditions.
What is a cell culture how can A cell culture be useful to biologist?
A cell culture is where cells are grown under controlled conditions.
What is the role of DMEM in cell culture?
It is the medium which contains all the required enriched nutrients essential for the cells to survive in invitro condition.
What is the role of Cloning Host in recombinant DNA technology?
The role of cloning host in RDT is to serve as the host cell under culture, in which the designed gene is subjected to produce recombinant protein.
How do you make large clear DMSO crystals?
DMSO (dimetylsulf oxide) (CH3)SO is a liquid with the freezing point at 19 oC.
