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Uñderstand by example: Bacteria that have pSC 101 plasmid, this plasmid have antibiotic resistant gene for tetracycline.when gene of interest in attached to plasmid to produce clone to get many genes of interest, it is placed in a medium contaning tetracycline for culturing bacteria(bactria made colonies which would separate out and remain safe because of they have resistance against tetracycline while other phothogen donot häve)
What else can I help you with?
Are molecular microbiology and recombinant gene technology the same?
no
How are cells screened to determine if the recombinant DNA was taken up?
Usually recombinant DNA is packaged in a plasmid that contains a marker gene. This marker can be an antibiotic resistance gene (NPTII for Kanamycin) or a gene that enables the plant to synthesise an amino acid. For antibiotic resistance the cells are grown on a medium that contains the antibiotic. The ones that grow have the marker gene. Sometimes the cells are transformed with a mixture of plasmids, some with the target gene and some without. The LAC-operon is used to select the cells that have the gene inserted. The gene-insertion inactivates the LAC-Z gene. Cells grown on X-gal plates will be blue, unless there's a transgene present. So white colonies have the transgene.
True or false cells containing a plasmid with an antibiotic resistance gene will die in the presence of the antibiotic?
False. Cells containing a plasmid with an antibiotic resistance gene will survive in the presence of the antibiotic because they can produce the protein that confers resistance, allowing them to withstand the antibiotic's effects.
Antibiotic in gene cloning?
If antibiotic resistance is added to the gene being cloned, antibiotics can be used to isolate the transformed bacteria (ones with the gene being cloned) by killing off all non-transformed bacteria, that don't have the antibiotic resistance. There is a chance that the non-transformed bacteria can mutate to develop antibiotic resistance.
What can gene for antibiotic resistance be used for?
genetic marker
What type of gene is used to distinguish bacteria that carry a plasmid containing foreign DNA from those that don' t?
The plasmid that contains foreign DNA is engineered to also carry an antibiotic resistance gene. This antibiotic resistance gene codes for a protein that is able to inactivate an antibiotic thus keeping the cell alive. In the absence of the antibiotic resistance gene, the cells would not survive when exposed to an antibiotic. After transfection (the process of inserting the plasmid carrying the foreign gene into cells), the cells are gown in media containing an antibiotic. Cells that contain the plasmid (and therefore contain the antibiotic resistance gene) are able to survive in this medium. Cells that do not contain the plasmid (and therefore lack the antibiotic resistance gene) do not survive in this medium. The process described above is called selection
A gene for antibiotic resistance may be used as?
Genetic Marker
Is it true cells containing a plasmid with an antibiotic resistance gene will die in the presence of the antibiotic?
false
Why do plasmids contain a gene for antibiotic resistance?
Plasmids contain antibiotic resistance genes because these genes provide a survival advantage to the bacteria in the presence of antibiotics. Bacteria can pick up plasmids with antibiotic resistance genes through horizontal gene transfer, allowing them to survive in environments with antibiotic exposure. This is a common mechanism for bacteria to acquire resistance traits and poses a challenge for antibiotic treatment.
How will you identify cells carrying the recombinant plasmid?
The transformants are selected for on agar containing an appropriate antibiotic. For example if your recombinant plasmid contains a kanamycin cassette, then only the cells containing the plasmid will grow on an agar plate containing kanamycin. PCR can then be performed on the colonies to ensure they contain your gene of interest on the plasmid.
Where does new DNA come from in gene cloning?
New DNA molecules can come from various sources in gene cloning, such as PCR amplification of a specific gene, synthesis of a gene using recombinant DNA technology, or isolation of a gene from a donor organism. These DNA molecules are then inserted into a vector, such as a plasmid, to create a recombinant DNA molecule for cloning.
Is cow or pig insulin made from their urine?
No it is made by their pancreas. Artifical insulin is made by recombinant DNA gene technology.
