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Usually recombinant DNA is packaged in a plasmid that contains a marker gene. This marker can be an antibiotic resistance gene (NPTII for Kanamycin) or a gene that enables the plant to synthesise an amino acid.
For antibiotic resistance the cells are grown on a medium that contains the antibiotic. The ones that grow have the marker gene.
Sometimes the cells are transformed with a mixture of plasmids, some with the target gene and some without. The LAC-operon is used to select the cells that have the gene inserted. The gene-insertion inactivates the LAC-Z gene. Cells grown on X-gal plates will be blue, unless there's a transgene present. So white colonies have the transgene.
What else can I help you with?
What step is not essential in producing recombinant DNA?
One non-essential step in producing recombinant DNA is incorporating a selection marker gene. While this can be useful for identifying cells that have successfully taken up the recombinant DNA, it is not absolutely necessary for the process of creating recombinant DNA itself.
Does recombinant DNA have something to do with plants?
Recombinant DNA is made of DNA taken from two different organisms. It may contain plant DNA but this is not always the case.
Blood cultures are taken to determine?
blood cultures are taken to determine
Will a picture taken on a small phone screen be larger if sent to a bigger screened phone?
Yes, but the quality wont be as good
What are bacteria taken into cells by?
Endocytosis.
How is bacteria taken into cells?
endocytosis
Have you ever taken an EMF sensitivity test to determine your sensitivity to electromagnetic fields?
No, I have not taken an EMF sensitivity test to determine my sensitivity to electromagnetic fields.
How do you determine the sex in wild ungulates?
Samples of DNA can be taken from feces to determine the sex.
What is the function of capillary net?
to spread the blood cells so thin that the oxygen can be taken into cells or transferred to other cells.
How are genetic markers related to transformation?
Genetic markers make it possible for researchers to mix recombinant plasmids with a group of bacteria. Add enough DNA to transform one cell into a million and still be able to "find" that cell.
What are vaginal specimens taken?
They are taken to check for cancer cells mainly but also STI's and other infections.
What provides new cells for growth and repair?
There are many vitamins and supplements that can be taken for growth and repair of tissues. Vitamin A is one example of a vitamin taken for growth and repair.
