Want this question answered?
The actual role of phenol chloroform isoamyl alcohol in a plasmid DNA extraction is to purify the DNA. The alcohol will act in part as a detergent.
It solubalize lipids and protiens to remove them from DNA
Chloroform denaturizes the proteins and facilitates the separation of the aqueous and organic phase. If needed the extraction with chloroform is performed two or three times to completely remove the impurities from aqueous layer..
Phenol and chloroform are very hydrophobic and are used to remove non-polar bio- molecules (proteins, fragments of membranes, lipids etc) in the extraction.
It solubilizes lipids and a lot of proteins to remove them from the DNA.
The actual role of phenol chloroform isoamyl alcohol in a plasmid DNA extraction is to purify the DNA. The alcohol will act in part as a detergent.
It solubalize lipids and protiens to remove them from DNA
to remove excess phenol from DNA to remove excess phenol from DNA
importance of phenol
Chloroform denaturizes the proteins and facilitates the separation of the aqueous and organic phase. If needed the extraction with chloroform is performed two or three times to completely remove the impurities from aqueous layer..
Phenol and chloroform are very hydrophobic and are used to remove non-polar bio- molecules (proteins, fragments of membranes, lipids etc) in the extraction.
It solubilizes lipids and a lot of proteins to remove them from the DNA.
chloroform is used to denature protein and settle it in the bottom during rna extraction ,also it helps to form organic and inorganic layers in which rna is dissolved in inorganic layer.
phenol is used in order to remove protein impurities from the DNA to yield pure dna while chloroform prevents shearing of DNA during isolation.
It plays a role.
roll of Na CL in DNA extraction
lyses cells