An essential cofactor for the DNA polymerase in PCR is Magnesium chloride. Its concentration must be optimized for every primer:template system. Many components of the reaction bind magnesium ion, including primers, template, PCR products and dNTPs. The main 1:1 binding agent for magnesium ion is the high concentration of dNTPs in the reaction. Because it is necessary for free magnesium ion to serve as an enzyme cofactor in PCR, the total magnesium ion concentration must exceed the total dNTP concentration. Typically, to start the optimization process, 1.5 mM magnesium chloride is added to PCR in the presence of 0.8 mM total dNTPs. This leaves about 0.7 mM free magnesium for the DNA polymerase. In general, magnesium ion should be varied in a concentration series from 1.5-4.0 mM in 0.5 mM steps.
I just read somewhere that some PCR reagents require free Mg2+
It helps to keep the enzymes such as Taq Polymerase in their optimal confirmation.
Magnesium chloride is an ionic salt. Hexane is a non polar solvent. So, magnesium chloride is unsoluble in hexane.
Magnesium Chloride is a compound. It contains Magnesium(Mg) and Chlorine(Cl). It is also a pure salt. Magnesium chloride is chemically bonded together.
hydrochloric acid + magnesium => magnesium chloride + hydrogen 2HCl + Mg => MgCl2 + H2
When Magnesium chloride is burnt in a Bunsen flame, it imparts no colour in the flame.
aqueous magnesium chloride is when magnesium oxide dissolves in hydrochloric acid. Aqueous means dissolved in water. ananya
mgcl2 acts as a cofactor for the DNA polymerase used and is necessary for efficient functioning of the pcr
It Inhibits the PCR reaction by chelating the magnesium ions.
MgCl2 is the chemical formula of magnesium chloride.
Magnesium+ Chloride= Magnesium ChlorideMg2+ + 2Cl1- = MgCl2the formula of magnesium chloride is MgCl2Magnesium and chlorine
I don't think you can have MgCl stable. MgCl2 is Magnesium Chloride.
Magnesium Chloride is MgCl2.
Magnesium chloride plus hydrochloric acid is magnesium chloride and hydrogen gas.
The products are magnesium chloride and tin
Magnesium chloride is already a compound.
Magnesium chloride is soluble in water.
Magnesium chloride (MgCl2) is a salt.
Magnesium chloride has ionic bonds.