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The most common use of a laser in an Atomic Force Microscope (AFM) is to generate a coherent beam of light that is reflected from the back of the AFM probe and onto a photodetector. As the AFM cantilever moves up and down, or twists left and right, the reflected beam traverses the photodetector creating a change in the voltage output from the segments (quadrants) of the photodetector. This difference signal is normalized to the total voltage output (the “sum”) and that normalized difference value is used as a measure of the degree of vertical deflection of the AFM probe. The coolest part of this system is the incredible sensitivity achieved by the laser behaving like a mechanical lever. Tiny motions of the AFM probe result in much larger motions of the laser traversing the photodetector. In this way one can measure nanoscale topographical changes or picoNewton forces on a surface. Lasers can also be used in the AFM to: cause thermal bending of AFM cantilever for remote mechanical control. heat the cantilever for thermal effects.

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Q: What is the role of the laser in an AFM experiment?
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