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Focusing a specimen under low power objective (LPO) allows you to find and center the specimen on the slide before moving to higher magnifications. This ensures that the sample is in the field of view and helps in adjusting the focus and moving to higher magnifications without losing sight of the specimen.
LPO stands for "Lowest Price Offer" and refers to the minimum price at which a buyer is willing to purchase a product or service. HPO stands for "Highest Price Offer" and refers to the maximum price at which a seller is willing to sell a product or service. Both terms are commonly used in negotiations and pricing strategies.
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The magnification of the specimen under low power optics, lpo, is 10 times and the magnification of the specimen under high power optics, hpo, depends on the power of the microscope but is usually at least 500 times or more.
In HPO, it has a rectangular shape and in LPO, it is elongated shape in LPO.
When viewed under the Low Power Objective (LPO), the image of the specimen appears larger and allows for a broader field of view, making it easier to locate and assess the overall structure and layout of the specimen. In contrast, the High Power Objective (HPO) provides a more magnified and detailed view, allowing for closer examination of specific features or cells within the specimen. However, the field of view is smaller under HPO, making it necessary to reposition the slide to observe different areas. Overall, LPO is useful for initial observations, while HPO is essential for detailed analysis.
LPO typically has a longer working distance compared to HPO, as LPO is designed for lower magnification levels and wider field of view, requiring the lens to be farther away from the specimen to capture the image. HPO, being designed for higher magnification levels, typically has a shorter working distance to achieve higher resolution and magnification.
The letter "e" is magnified twice under the LPO and twice again under the HPO, resulting in a total magnification of four times under both lenses.
The LPO will be shorter than HPO lens. LPO= magnifies 10x lenses HPO= magnifies 43x lenses
RAWR
The apical cells of hydrilla are elongated and cylindrical, forming the growing tip of the plant. Marginal cells are rectangular and arranged in a single file along the leaf margin, helping to provide structural support to the leaf.
When an objective lens in a light-path objective (LPO) microscope is shifted from the Low Power Objective (LPO) to the High Power Objective (HPO), the image becomes magnified and the field of view decreases. This results in higher magnification but a smaller area of the specimen being visible at once.
LPO (Low Power Objective) provides a wider field of view and lower magnification, making it suitable for locating objects and observing their overall structure. On the other hand, the higher magnification and narrower field of view of the HPO (High Power Objective) allow for more detailed examination of specific features or structures of the specimen. The scanner objective provides the lowest magnification and widest field of view for easily locating and navigating around the specimen on the slide.
When talking about the IPO and HPO, it is referring the magnification of a microscope. You can tell the difference of the two, because the LPO is shorter than the HPO.
The HPO (high-power objective) has a higher magnification than the LPO (low-power objective) in a microscope. This means that the HPO will show a smaller area but with more detail compared to the LPO.