The magnification of the specimen under low power optics, lpo, is 10 times and the magnification of the specimen under high power optics, hpo, depends on the power of the microscope but is usually at least 500 times or more.
LPO typically has a longer working distance compared to HPO, as LPO is designed for lower magnification levels and wider field of view, requiring the lens to be farther away from the specimen to capture the image. HPO, being designed for higher magnification levels, typically has a shorter working distance to achieve higher resolution and magnification.
The total magnification of a low power objective (LPO) is typically around 100x, a high power objective (HPO) is around 400x, and an oil immersion objective (OIO) can be up to 1000x. This means an oil immersion objective provides the highest magnification among the three.
LPO (Low Power Objective) provides a wider field of view and lower magnification, making it suitable for locating objects and observing their overall structure. On the other hand, the higher magnification and narrower field of view of the HPO (High Power Objective) allow for more detailed examination of specific features or structures of the specimen. The scanner objective provides the lowest magnification and widest field of view for easily locating and navigating around the specimen on the slide.
When talking about the IPO and HPO, it is referring the magnification of a microscope. You can tell the difference of the two, because the LPO is shorter than the HPO.
The letter "e" is magnified twice under the LPO and twice again under the HPO, resulting in a total magnification of four times under both lenses.
The HPO (high-power objective) has a higher magnification than the LPO (low-power objective) in a microscope. This means that the HPO will show a smaller area but with more detail compared to the LPO.
When an objective lens in a light-path objective (LPO) microscope is shifted from the Low Power Objective (LPO) to the High Power Objective (HPO), the image becomes magnified and the field of view decreases. This results in higher magnification but a smaller area of the specimen being visible at once.
When viewed under the Low Power Objective (LPO), the image of the specimen appears larger and allows for a broader field of view, making it easier to locate and assess the overall structure and layout of the specimen. In contrast, the High Power Objective (HPO) provides a more magnified and detailed view, allowing for closer examination of specific features or cells within the specimen. However, the field of view is smaller under HPO, making it necessary to reposition the slide to observe different areas. Overall, LPO is useful for initial observations, while HPO is essential for detailed analysis.
The advantage of LPO (Low Power Objective) is that it provides increased magnification and resolution for viewing smaller details on a specimen. The disadvantage is that it has a smaller field of view and less depth of field compared to lower magnification objectives. The advantage of HPO (High Power Objective) is that it offers even higher magnification for detailed examination of specimens. The disadvantage is that it has a very narrow depth of field, making it challenging to focus on thicker specimens or to view multiple layers simultaneously.
The LPO will be shorter than HPO lens. LPO= magnifies 10x lenses HPO= magnifies 43x lenses
RAWR
The light is typically brighter at the High Power Objective (HPO) compared to the Low Power Objective (LPO) in a microscope. This is because the HPO has a narrower field of view and higher magnification, allowing more light to be focused on a smaller area. However, the increased brightness at HPO may also depend on the microscope's illumination settings and the quality of the objectives used.