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Which has a longer working distance between LPO and HPO?
LPO typically has a longer working distance compared to HPO, as LPO is designed for lower magnification levels and wider field of view, requiring the lens to be farther away from the specimen to capture the image. HPO, being designed for higher magnification levels, typically has a shorter working distance to achieve higher resolution and magnification.
Compare the total magnification of lpo hpo and oio?
The total magnification of a low power objective (LPO) is typically around 100x, a high power objective (HPO) is around 400x, and an oil immersion objective (OIO) can be up to 1000x. This means an oil immersion objective provides the highest magnification among the three.
What is the difference of what you see under LPO or scanner with what you have observed under the HPO?
LPO (Low Power Objective) provides a wider field of view and lower magnification, making it suitable for locating objects and observing their overall structure. On the other hand, the higher magnification and narrower field of view of the HPO (High Power Objective) allow for more detailed examination of specific features or structures of the specimen. The scanner objective provides the lowest magnification and widest field of view for easily locating and navigating around the specimen on the slide.
How do you know which is lpo or hpo by just looking objective?
When talking about the IPO and HPO, it is referring the magnification of a microscope. You can tell the difference of the two, because the LPO is shorter than the HPO.
How much is the letter e you are now viewing under the scanner magnified under the LPO under the HPO?
The letter "e" is magnified twice under the LPO and twice again under the HPO, resulting in a total magnification of four times under both lenses.
What is the shape of the specimen under LPO and HPO?
Under Low Power Objective (LPO), the specimen typically appears larger and allows for a broader view of the sample, revealing overall structure and organization. In contrast, under High Power Objective (HPO), the specimen is magnified further, providing more detail about cellular structures and finer features, which can help in identifying specific characteristics of the specimen. The shape and arrangement of cells or tissues become more distinct at higher magnification, allowing for more precise observations.
Which is longer the HPO or LPO in the microscope?
The HPO (high-power objective) has a higher magnification than the LPO (low-power objective) in a microscope. This means that the HPO will show a smaller area but with more detail compared to the LPO.
What happens to the image of a focused object under LPO when the objective lens is shifted to HPO?
When an objective lens in a light-path objective (LPO) microscope is shifted from the Low Power Objective (LPO) to the High Power Objective (HPO), the image becomes magnified and the field of view decreases. This results in higher magnification but a smaller area of the specimen being visible at once.
How does the image of the specimen compare when viewed under the LPO and the HPO?
When viewed under the Low Power Objective (LPO), the image of the specimen appears larger and allows for a broader field of view, making it easier to locate and assess the overall structure and layout of the specimen. In contrast, the High Power Objective (HPO) provides a more magnified and detailed view, allowing for closer examination of specific features or cells within the specimen. However, the field of view is smaller under HPO, making it necessary to reposition the slide to observe different areas. Overall, LPO is useful for initial observations, while HPO is essential for detailed analysis.
What iis the advantage of lpo and hpo disanvantage?
The advantage of LPO (Low Power Objective) is that it provides increased magnification and resolution for viewing smaller details on a specimen. The disadvantage is that it has a smaller field of view and less depth of field compared to lower magnification objectives. The advantage of HPO (High Power Objective) is that it offers even higher magnification for detailed examination of specimens. The disadvantage is that it has a very narrow depth of field, making it challenging to focus on thicker specimens or to view multiple layers simultaneously.
When do we use lpo and hpo?
LPO (low power objective) and HPO (high power objective) are used in microscopy to examine specimens at different magnifications. LPO, typically around 4x to 10x, is ideal for scanning large areas and locating areas of interest, while HPO, usually 40x to 100x, allows for detailed observation of specific structures within the specimen. Utilizing both objectives helps provide a comprehensive understanding of the specimen's features.
How do you know which is LPO HPO of a microscope?
The LPO will be shorter than HPO lens. LPO= magnifies 10x lenses HPO= magnifies 43x lenses
