prosrdure of benidect solution test
It is normally benedicts solution. Add about 1/4 of the total sugar solution to a test tube and heat it in a hot water bath at around 50 degrees C for 5 min. For example, heat 40mL of sugar water (C12H22O11 aq ) with 10mL of Benedicts soluton (copper and sodium salts). If it is a positive test it will turn yellow.
A reducing sugar such as glucose can be oxidized by both Benedicts solution and Tollens reagent to form a colored precipitate. This reaction is used to test for the presence of reducing sugars in a solution.
Benedict's solution is commonly used to test for the presence of reducing sugars. When a reducing sugar is present, the solution changes color from blue to green, yellow, orange, or red, depending on the amount of sugar present.
Sodium carbonate is added to increase the pH of the solution.
Benedicts solution comprises of solution A(copper sulphate) and solution B(sodium pottasium citrate).chemically it is used as a test for aldehydes or generally carbonyl group.It is used to measure the sugar(allose,glucose,mannose,gullose,indose,galactose,tallose)content in food.-santa
Benedict's solution plus sugar is used in the Benedict's test to measure the presence of reducing sugars. The sugar reacts with the copper ions in Benedict's solution, forming a colored precipitate that indicates the presence of reducing sugars. This test is commonly used to detect the presence of glucose and maltose in various biological samples.
Most chemical supply companies sell it. You can do an internet search for "retail sale of Benedicts solution".
Benedicts solution
Benedict's solution is a chemical reagent used to test for the presence of reducing sugars, such as glucose. When heated with a reducing sugar, such as glucose, in the presence of an alkali (sodium carbonate), a redox reaction occurs, causing the blue color of the Benedict's solution to change to a brick-red precipitate of copper oxide. The intensity of the color change is proportional to the amount of reducing sugar present in the solution.
Benedict's solution is commonly used to test for reducing sugars in a sample. It reacts with reducing sugars such as glucose and fructose to produce a color change from blue to green, yellow, orange, or red, depending on the amount present in the sample.
The test for a reducing sugar using benedicts solution is by, first making a colour chart, using various KNOWN concentrations of glucose situations, if available. Then use the unknown substance and add benedicts solution and boil, a red precipitate should appear and the "redder" it is, the stronger the concentration. You can then filter off the precipitate and place the remaining liquid into a cuvette and pass through a colorimeter, on a red filter, the more light that passes through, the sronger the concentration. Compare this solution to your colour chart to help you determine the concentration of the reducing sugar. Thanks :) steph :)
Iodine solution is commonly used as a reagent to test for the presence of starch, not sugar. When iodine solution is added to a sample containing starch, it will turn blue-black if starch is present. Testing for sugar is typically done using reagents such as Benedict's solution or Fehling's solution.