What is tissu culture?

Answer 1

tissue culture you need cells from liver or organ

You finely chop them and you can create a primary cell line - which still has the 3d orientation of the cell. degrade it with trypsin and you have a continuous cell line. lasts 30 passages. You can immortalize it though it loses its liver key qualities. but still remains a cell non the less.

to maintain a tissue culture you need

7.4 pH

Nutrience

Glucose

Antibiotics

Serum

37 degrees