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You can use a simple Agarose gel to separate to view the DNA on electrophoresis. Use 0.8 - 1% gel for 5-10kbp , 2% for 0.2 - 1kbp.

If the fragments are really tiny, use an Acrylamide gel (vertical gel) to electrophorese and they will show right out. This is to offset the instability of high concentration gels.

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13y ago
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12y ago

the fluorescent dye is added in the DNA sample before loading in the gel.... after the gel is run, the gel is subjected to UV light as a result DNA is visualized.

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11y ago

S electable markers to track the DNA.

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11y ago

Gel electrophoresis

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Q: What is used to make the DNA visible on the gel?
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What makes DNA visible?

You can an electrophoresis gel and then stain the gel using a solution such as coomassie blue to make the bands visible. Alternatively, you can stain a cell containing DNA by using acridine orange. It is necessary to observe these under an electron light microscope.


Why stacking gel used in electrophoresis?

Stacking gel is used to make a thin uniform band of the DNa sample before the real seperation takes place.


How do you make DNA fingerprint?

gel electrophoresis


What is a gel used for in molecular biology research?

A"gel" generally refers to an agarose gel which is used to visualise DNA, determine the size of DNA and even a tell you a bit about its's structure (supoercoiled DNA vs linear DNA). However some gels can also be used to look at protein (often as a "western blot" gel) or RNA. The size of DNA, RNA or protein can be determined by how fast it moves across the gel when you pass an electrical current through it.


What is used to sort DNA into lengths?

Gel Electrophoresis


What is used to separate DNA fragments by size?

Electrophoresis. Restriction enzymes are used to cut DNA into fragments. Solutions containing these fragments are placed on the surface of a gel to which an electric current is applied. The electric current causes the DNA fragments to move through the gel. Because smaller fragments move more quickly than larger ones, this process separates the fragments according to size.


What is used to sort DNA into different lenghts?

Gel Electrophoresis


A technique that can be used to compare the DNA of two or more plants is?

Gel electrophoresis


What procedure is used to separate and analyze DNA fragments by placing a mixture of DNA fragments at one end of a porous gel and supplying an electrical voltage to the gel?

agarose gel electrophoresis


How could gel electrophoresis be used to tell whether a genetic modification experiment was successful?

Gel electrophoresis can be used to analyze differences in DNA before and after the genetic modification. In this process, the DNA on the gel moves according to size under the influence of an electric field. Changes in the size of the DNA after genetic modification can be seen on the gel


How does gel electrophoresis is used to make a DNA fingerprint?

Gel electrophoresis separates DNA fragment on the basis of their size. In DNA fingerprinting or DNA typing given sample is cut up with restriction enzymes and run through electrophoresis and results are analyzed to check for DNA polymorphism between the given sample and a sample form suspect. In nutshell gel electrophoresis is boon for the people in forensics.


Function of agarose in agarose gel electrophoresis?

Agarose is used in gel electrophoresis to separate nucleic acids (like DNA) by size, charge an other physical properties. Gel electrophoresis uses an electrical current to make particles move. For example, DNA is negative, so it'll travel towards to positive electrode of the gel box. Agarose has small pores through which a DNA can travel. Bigger fragments of DNA travel shorter distances, because it takes longer for them to navigate through the pores of the agarose gel. Identically sized pieces of DNA will travel the same distance, which is why you get bands (DNA with loading dye) after you run a a gel.