Since there might be problems with the specimen preparation.
The wavelength of the electron.
Resolving power is the ability of an imaging device to separate points of an object that are located at a small angular distance
.2 um
Short Answer:Electron microscopes have much better resolution and are capable of much higher magnification than light microscopes because the wavelength of the electrons is thousands of times smaller than the wavelength of light.Light microscopes can typically resolve structures to a fraction of a micron compared to electron microscopes which in practice achieve resolutions of a few nanometers. Practically, electron microscope can have almost a thousand times greater resolution than an optical microscope.The useful magnification of an electron microscope is also in the range of a thousand times greater than an optical microscope.The actual performance of any microscope depends on its design and lensing system and so significant variation exists in the above practical characteristics and performance of both types of microscopes can be enhanced in various ways.Long Answer:An electron microscope uses an electron beam to illuminate a specimen and produce the image.An optical or light microscope uses a light beam to illuminate a specimen and produce the image.A microscope of either type is characterized by its magnification and resolving power. The magnification depends on the lensing system and can be increased to any degree, but the maximum useful magnification is limited by the resolving power.The resolving power of a microscope can not be better than the limits placed on it by the size of the wavelength of the illuminating beam. The smaller the wavelength, the smaller the structures that can be resolved in them image.Visible wavelengths of light are a few hundred nanometers. An electron microscope operates with electrons accelerated to a few hundred thousand electron volts of energy and with a wavelength in the range of few hundredths of an Angstrom.An electron microscope has a theoretical resolving power that is much greater than a light microscope and can reveal smaller structures because the electrons used have wavelengths (few hundredths of Angstroms) almost 100,000 times shorter than visible light (few hundred nanometers).An optimized electron microscope can achieve a practical resolution of a few Angstroms and a useful magnifications in the millions of times.A good light microscope can resolve structures smaller than a micron but is limited to about a few hundred nanometers resolution. The useful magnification of a light microscope is not much more than a thousand times.The electron microscope uses electrostatic and electromagnetic fields to act as lenses to control and focus the electron beam and to form an image. An optical or light microscope employs glass lenses.
Transmitting Electron microscope has the greatest magnifying power.
The ability to distinguish close objects is called resolution or resolving power.It is important in microscopy as well as in other fields involving optical instruments, such as photography.A light microscope, using a high-power objective with oil immersion, can achieve a resolution of about 0.2 μm (micrometers). An electron microscope has, in practice, a resolving power about 100 times that, namely 1 to 2 nm (nanometers).
the resolving power of light microscope is 0.2 micrometr
A transmission electron microscope.
Electron microscopes don't have the resolving power to see individual molecules.
Resolving power is the ability of an imaging device to separate points of an object that are located at a small angular distance
A electron microscope uses a beam of electrons instead of light to magnify objects up to 500,000 times actual size. A electron microscope has much higher resolving power than light microscopes.
Resolving power of microscope is inversely related to the wavelength of the light used. So shorter the wavelength, greater the resolving power.
A virus of 50nm would be too small to see unless an electron microscope was used because it has greater resolving power and a resolution up to .1nm. A microscope using compound light as the means of illumination could not resolve better than approx. 200nms.
.2 um
No, you cannot see individual nucleotides through a microscope.With an electron microscope, it is just possible to make out some very large molecules (macromolecules), such as individual strands of DNA. But the resolution (resolving power) of even an electron microscope cannot distinguish free (uncombined) nucleotides.
The power source of an electron microscope is an electron gun located in the microscope that fires electrons through a series of lens and into a non-living object.
A virus of 50nm would be too small to see unless an electron microscope was used because it has greater resolving power and a resolution up to .1nm. A microscope using compound light as the means of illumination could not resolve better than approx. 200nms.
Around 0.2micrometers or 200 nm