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The fine focus knob is used to bring an object into fine focus, while the course focus knob is used to bring an object into approximate focus. The course focus knob should only be used on the low-power and scanning objectives of the light microscope. Using it on the high power microscope is okay, but you should be very careful. It is no recommended to use the course adjustment knob on the oil-emersion objective as it is so close to the stage and could lead to a potential break in the slide. Fine focus adjustment knob is useful to bring out the fine details in the specimen on your slide. Can be used on any of the objectives, but most receptive on the high-power and oil-emersion objectives.

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Why do we never use the coarse focus knop while the high power 40x lens in place?

We avoid using the coarse focus knob with the 40x lens because it can lead to a high risk of crashing the lens into the slide, potentially damaging both the slide and the objective lens. The coarse focus knob moves the stage significantly, which is suitable for lower magnifications, but at high power, even small movements can cause contact. Instead, the fine focus knob should be used to make precise adjustments for clear imaging.


What is the knob you turn first to bring s slide to focus?

You use the coarse adjustment first.


Proper use of microscope in 400 magnification?

Oculars are generally 10x, so I assume you're talking of the 40x objective (10x40=400, how total magnification is derived). The most important rule is NEVER touch the coarse focus adjustment, only the fine; you run the risk of breaking the specimen or the objective if you mash the two together. Other things to consider- high light level, make sure koehler illumination is established and adjust your field aperture to eliminate refringence.


What happens if you try to use the coarse adjustment when the 10x lens is in place?

One thing that can happen if you use the coarse adjustment while the oil immersion objective is in place is that the slide will end up breaking. It is better to use the fine adjustment.


What part of the microscope should you used to focus the image when using high power objective lens?

First of all, one should never call it high power, it is morecommonly called the "high objective", yet that is not what this question is asking. To answer the question: You use the fine adjustment knob. This knob should be located near the coarse adjustment knob, on the opposite side of the microscope (at the same height as the coarse adjustment knob), or even as a separate knob protruding from the coarse adjustment knob. The fine adjustment knob is smaller in size. You should never, ever use the coarse adjustment knob under the high objective, you could scratch the microscope slide, cover slip, high objective lens, or in a worst case scenario break the high objective lens.

Related Questions

What is used to focus when using the scanner and low objective?

When using the scanner and low objective on a microscope, you should use the coarse focus knob to bring the specimen into approximate focus and then use the fine focus knob to achieve a sharp image. Adjusting the height of the stage may also be necessary to fine-tune the focus.


What 2 structures on the microscope will you use to focus on your specimen?

To focus on your specimen, you will typically use the coarse adjustment knob for initial focusing and the fine adjustment knob for fine-tuning the focus. These two knobs allow you to move the objective lens closer to or further from the specimen to achieve a sharp image.


What are the parts of microscope and its use?

The main parts of a microscope include the eyepiece, objective lenses, stage, light source, and coarse/fine focus knobs. The eyepiece is where you look into the microscope, while the objective lenses magnify the specimen. The stage holds the specimen in place, and the light source illuminates it. The coarse and fine focus knobs are used to adjust the focus of the specimen. Microscopes are used to magnify and view tiny objects that are not visible to the naked eye.


Fine adjustment of microscope?

To make fine adjustments on a microscope, use the fine focus knob to sharpen the image. This knob allows you to bring the specimen into clear focus by moving the objective lens slightly up or down. Use it after using the coarse focus knob to roughly focus the image. Make small, careful movements until the specimen is sharply in focus.


What is the procedure for focusing a microscope using the coarse and fine adjustment knobs?

To focus a microscope using the coarse adjustment knob, gently turn it in one direction until the specimen comes into view. Then, use the fine adjustment knob to further sharpen the image. Avoid using excessive force on either knob to prevent damaging the microscope or specimen.


What two structure on the microscope will you use to focus on your specimen?

First use the coarse focus followed by the fine focus.


Which knob on a microscope do you use to focus on low power?

You would use the coarse focus knob to focus on low power. Turn the coarse focus knob clockwise to bring the specimen into view and then fine-tune using the fine focus knob.


What structures on the microscope will you used to focus on your specimen?

First use the coarse focus followed by the fine focus.


What is the name of the control knob used for sharp focusing a compound microscope?

There are two knobs. The "coarse focus" knob changes the focal length very quickly, so you use that to get a close to sharp image. Then, you use the "fine focus" knob to perfect the image. Both knobs do the same thing, just at different rates. Resource: http://shs.westport.k12.ct.us/mjvl/biology/microscope/microscope.htm


What is fine adjustment of the microscope?

You use the fine adjustment to focus in HIGH power with a compound microscpe.


What is coarse focus used for?

Coarse focus on a microscope is used only when you are using the lowest objective lens. You use it to get the object magnified mostly. The fine focus is then used to adjust the resolution to the viewer's eyesight.


What two structures on the microscope will you use to focus on your specimen?

To focus on a specimen using a microscope, you will typically use the coarse adjustment knob for initial focusing and the fine adjustment knob for precise focusing. The coarse adjustment knob moves the stage up and down quickly to bring the specimen into view, while the fine adjustment knob allows for small, precise adjustments to achieve a clear image.