You use the coarse adjustment first.
When using any high power objective, you should not adjust the coarse focus knob. This is because the high power objective lens is positioned very close to the slide, and adjusting the coarse focus can lead to crashing the lens into the slide, potentially damaging both the lens and the specimen. Instead, use the fine focus knob for precise adjustments to bring the specimen into sharp focus.
The fine focus knob is used to bring an object into fine focus, while the course focus knob is used to bring an object into approximate focus. The course focus knob should only be used on the low-power and scanning objectives of the light microscope. Using it on the high power microscope is okay, but you should be very careful. It is no recommended to use the course adjustment knob on the oil-emersion objective as it is so close to the stage and could lead to a potential break in the slide. Fine focus adjustment knob is useful to bring out the fine details in the specimen on your slide. Can be used on any of the objectives, but most receptive on the high-power and oil-emersion objectives.
The coarse focus knob on a microscope is used to make large adjustments to the focus of the specimen being observed. It moves the stage or the objective lens significantly, allowing you to quickly bring the sample into general focus. After using the coarse focus to get a rough image, you should switch to the fine focus knob for more precise adjustments and clearer details. Always start with the lowest power objective lens when using the coarse focus to avoid damaging the slide or lens.
The coarse adjustment knob is only used with the low-power objective lens (usually 4x) because all the other ones are too long and the coarse adjustment knob may bring the slide up too qucikly, therefore it might break the slide or crush the specimen being observed. When using the medium (10x) and high (40x) power objective lens, it is best to use the fine adjustment knob to focus on the details instead.
The coarse adjustment knob is used to raise or lower the body tube to bring the specimen into general focus.
When you first begin looking at a slide under a microscope, you should use the coarse focus knob. This knob allows for quick adjustments to bring the specimen into general focus. Once the specimen is roughly in focus, you can switch to the fine focus knob for more precise adjustments.
The fine adjustment knob is used to bring the image of the object on the slide into focus. It allows for small, precise movements of the lens to sharpen the image.
You do not use the coarse focus knob on high power because it can damage the slide and the objective lens of the microscope. Use only the fine focus knob to bring the specimen into sharp focus on high power.
The fine adjustment knob is used to bring the image of an object on the slide into focus when under the high power objective lens. This knob allows for precise focusing by making small adjustments to the distance between the objective lens and the specimen on the slide.
The coarse adjustment knob should be used first to bring the specimen into focus at the lowest magnification. It allows for larger adjustments in focus compared to the fine adjustment knob.
To adjust the slide when the specimen is out of the field of view to the top, gently move the slide on the stage towards you using the x and y-axis controls on the microscope. Start by adjusting the coarse focus knob to bring the specimen roughly into view, then fine-tune the focus with the fine focus knob until the specimen is in focus at the top of the field of view.
To focus a microscope from 4x to 40x, start by using the 4x objective lens. Position the slide on the stage and use the coarse focus knob to bring the specimen into view, adjusting the light as needed. Once focused, switch to the 10x objective lens, using the fine focus knob to sharpen the image. Finally, switch to the 40x lens, again using the fine focus knob for optimal clarity, ensuring not to use the coarse knob at this magnification to prevent damage to the slide or lens.
Start by placing the slide on the stage and securing it with the stage clips. Begin with the lowest magnification objective lens and adjust the coarse focus knob to bring the specimen into general focus. Use the fine focus knob to bring the specimen into sharp focus by making small adjustments. Finally, switch to higher magnification lenses and repeat the focusing process as needed.
The rough focus knob is what makes the contents of the slide visible. Once you get it as visible as you can with the rough focus knob, you switch to the fine adjustment and see if you can make the image clearer.
When focusing the object under low power, you should use the coarse focus knob first to bring the object into general focus. Once the object is roughly in focus, then you can use the fine focus knob to sharpen the image.
The coarse adjustment knob should be used first to bring the specimen into general focus. Then, the fine adjustment knob can be used for precise focusing.
You would use the coarse focus knob to focus on low power. Turn the coarse focus knob clockwise to bring the specimen into view and then fine-tune using the fine focus knob.