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What is the component in the SIM deep tubes that makes this medium suitable to detect the production of indole?
Wiki User
∙ 10y ago
The ingredients in SIM Medium enable the determination of three activities by which enteric bacteria can be differentiated.
Sodium thiosulfate and ferrous ammonium sulfate are indicators of hydrogen sulfide production. The ferrous ammonium
sulfate reacts with H2S gas to produce ferrous sulfide, a black precipitate.1 The casein peptone is rich in tryptophan, which is
attacked by certain microorganisms resulting in the production of indole. The indole is detected by the addition of chemical
reagents following the incubation period. Motility detection is possible due to the semisolid nature of the medium. Growth
radiating out from the central stab line indicates that the test organism is motile.
Wiki User
∙ 13y ago
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What is the component in the SIM deep tubes that makes the medium suitable to detect the production of indole by bacteria?
Tryptophan
What ingrediant could be eliminated if the medium was strictly used for indole production?
Trytophan
What is a MIO test?
Motility, Indole production and Ornithine decarboxylase activity(MIO) Used for identification and differentiation of bacteria.
Why determine motility and h2s before adding kovac's reagent?
You will do this regardless since Indole is part of the SIM media....this media will turn black if H2S is present and you read it for motility just like standard motility media. It must incubate for 24-28 hrs before doing the indole portion. Hope this helps.
What are the indole test principles?
this test is done to determine if bacteria can breakdown the amino acid tryptophan into indole
What is the component in the SIM deep tubes that makes the medium suitable to detect the production of indole by bacteria?
Tryptophan
What is Staphylococcus aureus sim test results?
Staphylococcus aureus is negative for motility, and positive for the indole production. The SIM test tests for sulfur reduction, indole production and motility. Positive for indole production means tryptophan is broken down into indole and pyruvate and will give a red color. Motility, you will see the bacteria move outside of the stab. Positive for sulfur reduction will give a black color in the medium.
What factors dictate the choice of test in a combination medium?
Hydrogen Sulfide production, Motility, and Indole production.
What ingrediant could be eliminated if the medium was strictly used for indole production?
Trytophan
What factors dictate choice of tests included in a combination medium?
The hydrogen sulfide production, motility, and indole production.
What factors dictate the choice of tests included in a combination medium?
The hydrogen sulfide production, motility, and indole production.
Explain the chemical mechanism for detecting indole in a bacterial culture?
Indole is extracted from the medium and into the reagent layer (Kovac's) by the acidified butyl alcohol component and forms a complex with the p-dimethylaminobenzaldehyde, yielding the cherry red color seen when the culture is indole positive.
Does proteus vulgaris produce h2s?
Yes, it does. It is Indole Production positive, H2S positive, and Motility positive. This can all be seen using SIM agar. Yes, it does. It is Indole Production positive, H2S positive, and Motility positive. This can all be seen using SIM agar.
What is a MIO test?
Motility, Indole production and Ornithine decarboxylase activity(MIO) Used for identification and differentiation of bacteria.
Is staphylococcus epidermidis indole positive?
S.aureus is a positive gram. Indole test is a part the biochemistry test to determinate a bacteria negative gram. indole test used to determine S.aureus, the result is negative. That is improve which S.aureus haven't trytophanase enzym (please see indole test principle)bcb
What is an indole alkaloid?
class of alkaloids containing a structural moiety of indole.
Why determine motility and h2s before adding kovac's reagent?
You will do this regardless since Indole is part of the SIM media....this media will turn black if H2S is present and you read it for motility just like standard motility media. It must incubate for 24-28 hrs before doing the indole portion. Hope this helps.
