Prolonged heating of the slide can cause the slide to shatter.
I'm sorry but you have to specify your question. What kind of slide? What degree of flame? WHat is the base of the flame emitting from?
It will crack or break.
You will cook it!
A Gram stain refers to a staining technique for the identification of bacteria. A Gram stain done on a slide that was heated too hot during the heat-fixed smear will destroy the cell wall of the bacteria.
You can prevent a smear from accidentally washing-off a slide by using heat to fix the cells to the slide and it can be achieved by following this procedure:Allow the smears to air dry first.Fix the cells to the slide by gently heating.Hold the slide on the frosted end with your clothespin found in your cabinet.Do this by quickly passing the underside of the slide over the flame of a Bunsen burner.Each time, touch the slide to the back of your hand - when it becomes uncomfortable, it is done.
It dries the smear and fixes the cells to the slide
Prolonged heating of the slide can cause the slide to shatter.
Any heat much over room temperature at that point would denature the cells due to the water content on the slide. Even if it was gentle, the heat from the flame would be enough to sizzle them like tiny fried eggs. :)
It will crack or break.
heat fixing
You will cook it!
I guess so, and that's why you put out the flame after that.
Slide indicator (crossword problems?)
a slide derule
a slide rule
A Gram stain refers to a staining technique for the identification of bacteria. A Gram stain done on a slide that was heated too hot during the heat-fixed smear will destroy the cell wall of the bacteria.
You can prevent a smear from accidentally washing-off a slide by using heat to fix the cells to the slide and it can be achieved by following this procedure:Allow the smears to air dry first.Fix the cells to the slide by gently heating.Hold the slide on the frosted end with your clothespin found in your cabinet.Do this by quickly passing the underside of the slide over the flame of a Bunsen burner.Each time, touch the slide to the back of your hand - when it becomes uncomfortable, it is done.
A smear preparation is a techniques that is used as a precursor is many different staining techniques.The smear prep usually calls for the sample bacterium to be placed on a microscope slide (use aseptic technique) with one drop of DI water. Then the slide is allowed to air dry for a few minutes then the slide is passed through a Bunsen burner flame. Use a cloths pin so that you do not burn you hands Only pass the glass microscope slide through the flame not you cloths pin if it is wood it will catch fire. Pass the slide through 3-4 times then you are ready to continue on with your desired staining technique