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What problems may arise in the use from a poorly packed liquid chromatography column?
Wiki User
∙ 11y ago
The effect of packing a chromatographic column unevenly will cause the flow to be uneven down the column and lead to poor separation.
Wiki User
∙ 9y ago
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What difference between dad and uv detector in hplc?
Liquid chromatography (LC) encompasses all chromatographic techniques using liquid mobile phase, including planar chromatography (paper chromatography and thin-layer chromatography) and column chromatography (classical column chromatography, and high-performance liquid chromatography on packed and capillary columns). The term liquid chromatography is nowadays often used as a sinonim for high performance liquid chromatography (HPLC) and ultra-high performance liquid chromatography (UHPLC).
What is void volume in chromatography?
bed volume= pie*r square*h where, pie=3.14 r=radius of column h=height of column it is the total volume of column packed with the gel.
How high-performace liquid chromatography works?
High-performance liquid chromatography (HPLC) works by pumping a liquid sample through a column packed with tiny particles. These particles have different affinities for the components of the sample, causing them to separate as they pass through the column. The separated components are then detected by a detector, which produces a chromatogram. HPLC is commonly used in analytical chemistry to separate and quantify compounds in a mixture.
How does chromatography work to separate particles?
When a solvent diffuses, it 'drags' whatever is dissolved in it along with it. The further it d\e same, the different dissolved materials sort out as different stripes or peaks and the banding can t\tography
Why do open tubular columns provide greater resolution than packed columns in gas chromatography?
Open tubular columns provide greater resolution than packed columns in gas chromatography because of their large retention time.
What difference between dad and uv detector in hplc?
Liquid chromatography (LC) encompasses all chromatographic techniques using liquid mobile phase, including planar chromatography (paper chromatography and thin-layer chromatography) and column chromatography (classical column chromatography, and high-performance liquid chromatography on packed and capillary columns). The term liquid chromatography is nowadays often used as a sinonim for high performance liquid chromatography (HPLC) and ultra-high performance liquid chromatography (UHPLC).
What is the efficiency of packed columns in gas chromatography?
It is around 4000-6000 plates for a 2 meter column.
What is void volume in chromatography?
bed volume= pie*r square*h where, pie=3.14 r=radius of column h=height of column it is the total volume of column packed with the gel.
What is loading point in packed column?
The point in which the droplets of liquid (solvent) are carried up with the gas in packed column. This hapens if the solvent flow is fixed & the gas flow is icreased.
How high-performace liquid chromatography works?
High-performance liquid chromatography (HPLC) works by pumping a liquid sample through a column packed with tiny particles. These particles have different affinities for the components of the sample, causing them to separate as they pass through the column. The separated components are then detected by a detector, which produces a chromatogram. HPLC is commonly used in analytical chemistry to separate and quantify compounds in a mixture.
What are the advantages and limitations of column chromatography over thin layer chromatography?
TLC. The mobile phase is a liquid, the stationary phase is a solid. Useful for seperating and comparing mobility of solids and some liquids dissolved in the mobile phase by their affinities to the solid phase relative to the mobile phase. GLC. The mobile phase ia s gas, the stationary phase is a liquid on a solid support. same concept as TLC. useful for seperating gases by their affinities to the stationary phase...the mobility can then be compared to known compounds for possible identification.
What is advantage of packed column?
no
How does chromatography work to separate particles?
When a solvent diffuses, it 'drags' whatever is dissolved in it along with it. The further it d\e same, the different dissolved materials sort out as different stripes or peaks and the banding can t\tography
Why do open tubular columns provide greater resolution than packed columns in gas chromatography?
Open tubular columns provide greater resolution than packed columns in gas chromatography because of their large retention time.
What carrier gas is used in packed columns of gas chromatography?
Nitrogen is usually used.
How do you convert centimeter to L?
Centimeter is a measurement of length. L - assuming you mean "liter" is a measurement of volume, which is length cubed. L - assuming you mean column length, is the length of chromatography column in HPLC or capillary in CE used to perform the liquid-phase separation, which can be measured in centimeters. Note that in some cases the actual column may be longer than the portion that is performing separation, i.e. some of the column may be effectively "empty" and should not be used in calculations requiring the length over which separation is occurring. In that case, only the packed or lined portion of the column should be used for the measurement.
Why the pressure drop is low in packed column?
air reduction
