Spirochaetes are best seen under darkfield microscopy.
Oil Red O stain is useful for visualizing chylomicrons in tissues, as it stains lipids red and helps in their identification under a microscope.
The dapi nuclear stain is highly effective in visualizing cellular nuclei in fluorescence microscopy.
To differentiate between oral streptococci, yeast, and spirochaetes in a sample, you can use appropriate staining techniques such as Gram stain or special stains like periodic acid-Schiff (PAS) stain for yeast. Additionally, you can utilize microscopy to observe the morphology and arrangement of the microorganisms. Each group will have distinct characteristics - streptococci appear as gram-positive cocci, yeast as larger round cells, and spirochaetes as spiral-shaped bacteria.
Spirochaetes is a phylum of distinctive Gram-negative bacteria, which have long, helically coiled cells.[1] Spirochetes are chemoheterotrophic in nature, with lengths between 5 and 250 µm and diameters around 0.1-0.6 µm.[citation needed] Spirochaetes are distinguished from other bacterial phyla by the location of their flagella, sometimes called axial filaments, which run lengthwise between the cell membrane and outer membrane. These cause a twisting motion which allows the spirochaete to move about. When reproducing, a spirochaete will undergo asexual transverse binary fission
Spirochaetes is a class which belongs in the phylumSpirochaetae.All "spirochaetes" belong to the same order - so they are members of both the phylum and the class.
india ink
A confocal laser scanning microscope is most useful for visualizing biofilms because it provides detailed three-dimensional images of the biofilm structure. Its ability to create optical sections at different depths within the biofilm allows for a better understanding of its architecture and spatial distribution of cells.
Fontana silver staining.
Basic dyes are a type of dye that are positively charged and primarily used to stain acidic components in cells and tissues in biological samples for microscopic examination. They have a strong affinity for structures that are negatively charged, such as nucleic acids and proteins, making them useful for visualizing these components under a microscope.
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Spirochaetes bacteria move by rotating and flexing their flexible, helical-shaped body. They use special appendages called axial filaments or endoflagella, which are located underneath the outer sheath, to generate the twisting motion. This unique flagellar arrangement enables spirochaetes to move through viscous environments, such as bodily fluids or mucus.
(Please elaborate:)FACTORSUPHOLSTERY/FABRIC TYPE: how this effects the stain removal methodORGANIC/INORGANIC: which types of stain removers are more common for organic stains?PH: Acidity of stain and stain remover, chemical reactions involvedTEMPERATURE: when cold is useful to keep stains from setting? when heat aids chemical reactions for stain removal? (steam,)TIME: time for a stain to set; for a detergent to soak or workScrubbing/Rubbing: When to do so and pressure exertedMECHANISMSALKALINES (,baking soda): are useful when... and do this:,ACIDS (,vinegar): are useful when...Water: for surfactant/carrying agent/dilution of detergentSurfactants: wetting, dispersing, emulsifying, foamingPeroxide:Ammonia:Bleaches:Enzymes:Other types of cleaners: commercial, cleaners for inorganic stains; for special materials or stains, steam