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Yeast doesn't need a substrate. All it needs are the optimum conditions.

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13y ago

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Howdy there! Negative control for a fermentation lab should have the substrate in the vessel, and it should be very sterile so no contamination doesn't happen (make sure to label them vessels well). Secondly, the negative control vessel should not be inoculated with the microbe, like yeast, in it at all because you want no response. If there is a response, it could be contamination or you mistakenly inoculated with the microbe which fermented the substrate. Hope this helped you plenty! 😄


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