What test show hydrolysis of proteins?
Because proteins are polymers of amino acids, hydrolysis followed by amino acids determination is a method. Ninhydrin is an oxidating agent which leads to the oxidative deamination of alpha-amino groups. Ninhyndrin test is used to detect ammonia, primary (deep blue or purple color (Ruhemann's purple)) and secondary amines (yellow), this is a proof of free amino acids in proteins. to distinguisn between Amino Acids and proteins you need a peptide bond test which can be…
How are the processes of dehydration synthesis and hydrolysis related to the organic compuounds such as carbohydrates lipids and proteins?
Proteins are more resistant to acid hydrolysis than are nucleic acids. Because proteins contain long chains of the amino acids which are connected to each other. -OH and -NH2 group of amino acids are strongly stabilised by hydrogen bonding. Therefore they do not get hydrolysed as number of intermolecular and intramolecular hydrogen bonds are formed.
Amino acids become proteins through the process of dehydration synthese. On other hands, proteins are broken down into amino acids through the process of hydrolysis. Def. of Hydrolysis: chemical decompostition in which a compound is split tinot other compounds by reacting to water Def. of Dehydration Synthesis: taking water out of a molecule to build it up
Many biomolecules, such as proteins, carbohydrates, and lipids are broken down into their smaller components by a process called hydrolysis, which means "water splitting." In hydrolysis, biomolecules break down by the addition of water molecules. Refer to the related link for an example of the hydrolysis of sucrose (table sugar).
Acid-base-catalysed hydrolyses are very common; one example is the hydrolysis of amides or esters. Their hydrolysis occurs when the nucleophile (a nucleus-seeking agent, e.g., water or hydroxyl ion) attacks the carbon of the carbonyl group of the ester or amide. In an aqueous base, hydroxyl ions are better nucleophiles than polar molecules such as water. In acids, the carbonyl group becomes protonated, and this leads to a much easier nucleophilic attack. The products for both…