20e API kit
Proteus is phenylalanine deaminase, urease and indole positive Salmonella negative. Proteus swarm on nonselective media, Salmonella does not.
Shigella species are generally indole negative, except for Shigella flexneri, which can be indole positive depending on the specific strain. In laboratory identification, indole testing is used to differentiate between various enteric bacteria. Therefore, while most Shigella species do not produce indole, Shigella flexneri may show positive results under certain conditions.
A simple test you can perform is the Citrate test, Serratia marcensens is Citrate positive while Shigella flexneriis citrate negative.Also Serratia Marcensens is motile and Shigella flexneri is non motile if you have already done a Hydrogen Sulfide test you would have been able to already determine between the two.Also Serratia marcensens is VP positive while Shigella flexneri is negative.Serratia marcensens is lipase positive and Shigella flexneri is lipase negativeAnother distinguishing characteristic is that Serratia marcensens grows a distinct red color pigmentation the Shigella flexneri does not have.(These are all according to Bergeys Manual of Systematic bacteriology)
The TSI (Triple Sugar Iron) test is commonly used to differentiate between members of Enterobacteriaceae family, such as Salmonella, Shigella, and E. coli based on their ability to ferment lactose, sucrose, and produce hydrogen sulfide gas. It is particularly useful in identifying pathogens causing foodborne illnesses.
Blood agar is a differential media since you can differentiate between different organisms based on the colony appearance. In a blood agar you are looking for hemolysis.
The biochemical test commonly used to identify Salmonella typhi is the Widal test. This test detects antibodies produced in response to a Salmonella typhi infection by measuring agglutination (clumping) reactions between the bacteria and specific antibodies in the patient's serum. Other tests like blood culture and polymerase chain reaction (PCR) can also be used for confirmation.
differentiate between general and specific reserve?
It's important to differentiate between right and wrong. She could not differentiate between the two identical twins. The new software features were designed to help differentiate their product from competitors. The expert could easily differentiate authentic artwork from fakes.
Differentiate a pilot and a plane!
If the meat was between 135°F and 70°F during those three hours and was contaminated, then it is possible that the meat could have grown an infective dose of Staph aureus, Salmonella, E. Coli, Listeria monocytogenes, or Shigella.
Differentiate between a simple bridge and transparent bridge