imvic test helps in identi fication of salmonello typhi and a specific agar named salmonella shigella agar is used for culturing these species
In a Widal test, "H" refers to the flagella antigen of Salmonella typhi while "O" refers to the somatic antigen of the bacteria. The presence of antibodies against these antigens indicates a recent or current infection with Salmonella typhi, the bacterium that causes typhoid fever.
The blood test for typhoid is called the Widal test. It detects antibodies in the blood against the Salmonella typhi bacteria, which causes typhoid fever.
A biochemical test is a test used mainly in microbiology with the main intention of detecting enzyme production. Biochemical tests can also, when used correctly, further narrow the search when looking at identifying an unknown microbe. The biochemical tests can narrow the search down to a specific genus and possibly even the species of the unknown microbe. In most cases when identification of an unknown microbe is needed, the microbe will usually be a bacterium.
Biochemical tests are generally not used for the identification of viruses. Instead, techniques such as serological assays, nucleic acid amplification tests (PCR), electron microscopy, and viral culture methods are commonly used to identify viruses. These methods help to detect specific viral proteins or genetic material in samples.
Serial dilution in Widal test helps to determine the titer of antibodies in the patient's serum by observing the agglutination reaction with specific antigens. By diluting the serum sample in a stepwise manner, the titer at which agglutination occurs can be determined, providing valuable information about the presence and concentration of antibodies against Salmonella typhi and Paratyphi antigens in the blood.
it should test + for catalase but Salmonella isolates are moderate catalase reactors.
If a blood test comes back with salmonella typhi O at a level of 1 80, it means a person has contracted typhoid fever.
In a Widal test, "H" refers to the flagella antigen of Salmonella typhi while "O" refers to the somatic antigen of the bacteria. The presence of antibodies against these antigens indicates a recent or current infection with Salmonella typhi, the bacterium that causes typhoid fever.
A positive Widal test result for Salmonella Typhi O indicates the presence of antibodies against the O antigen of Salmonella Typhi, suggesting a possible active or recent infection with typhoid fever. However, this test may yield false positives due to cross-reactivity with other infections or previous vaccinations, so it should be interpreted alongside clinical symptoms and other diagnostic tests. Additionally, the test is more reliable in endemic areas where typhoid fever is common.
The blood test for typhoid is called the Widal test. It detects antibodies in the blood against the Salmonella typhi bacteria, which causes typhoid fever.
the most commonly used biochemical test to identify lactobacillus is carbohydrate fermentation, this is the preliminary screening test and an essential test for the identification of lactobacilli
salmonella typhi H is a typhoid count. it is very dangerous. in typhi H it tells us that the count of the disease will decrease very slowly and it will take years time to decrease but in typhi O it tells us that the count will decrease in a short period of time such as in 1 or 2 weeks. the count 1/80 tells we are at a minimum level. that means we have typhoid tYphi O,but if its above the count it is very bad. if you have temprature or shiviring and vomitting you should immediatly go to the doctor for a blood test to ensure safety. remember one thing if you have typhoid you should take a diet.do not eat anything solid only take liquids.such as soups. i have also got typhoid and this is what he doctor said to me and i had typhi O.and in typhoid you should take rest for at least a week.
Indole - NegativeMethyl Red - PositiveVouges Proskauer - NegativeCitrate-NegativeTSI - Alkaline slant/Acid butt with H2SSIM - Motile, H2S positiveUrease - Negative
You can differentiate between different salmonella strains using serology. Each strain has its own combination of antigens. Depending on the O, H, and vi antigens, you can determine which strain is present. The O-antigen is the somatic polysaccharide antigen, the H-antigen is the flagellar antigen and the vi-antigen is the capsular antigen. Antibodies to most of the variants of these antigens are available commercially for use in labs. Adding an aliquot of specific antisera to a suspension of your salmonella spp, and incubating it for a few hours in a water bath will result in visible agglutination if your salmonella strain possesses the specific antigen to the antibody you added. For example, Salmonella typhi will show agglutination with O [9,12], H [d] and vi.
mywadil test is 1/160 of Para - typhi AH
The most common biochemical tests are gram stain, oxidase, catalase and coagulase tests. However, there are literally hundreds of biochemical tests that are commonly used to identify bacteria. For further information, check out MicrobeID.com, where you can find identification methods, keys, probabilistic databases, selective and differential media guides, as well as book reviews releated to bacterial identification. I would also recommend Bergey's Manual of Deterministic Bacteriology.
A biochemical test is a test used mainly in microbiology with the main intention of detecting enzyme production. Biochemical tests can also, when used correctly, further narrow the search when looking at identifying an unknown microbe. The biochemical tests can narrow the search down to a specific genus and possibly even the species of the unknown microbe. In most cases when identification of an unknown microbe is needed, the microbe will usually be a bacterium.